Please use this identifier to cite or link to this item: https://doi.org/10.1107/S0907444903011648
DC FieldValue
dc.titlePurification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus
dc.contributor.authorLee, K.-C.
dc.contributor.authorLim, D.
dc.contributor.authorWong, S.-M.
dc.contributor.authorDokland, T.
dc.date.accessioned2014-10-27T08:37:57Z
dc.date.available2014-10-27T08:37:57Z
dc.date.issued2003-08-01
dc.identifier.citationLee, K.-C., Lim, D., Wong, S.-M., Dokland, T. (2003-08-01). Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus. Acta Crystallographica - Section D Biological Crystallography 59 (8) : 1481-1483. ScholarBank@NUS Repository. https://doi.org/10.1107/S0907444903011648
dc.identifier.issn09074449
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101508
dc.description.abstractHibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 Å, and diffracted X-rays to at least 4.5 Å resolution.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1107/S0907444903011648
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1107/S0907444903011648
dc.description.sourcetitleActa Crystallographica - Section D Biological Crystallography
dc.description.volume59
dc.description.issue8
dc.description.page1481-1483
dc.description.codenABCRE
dc.identifier.isiut000184322700025
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

9
checked on Nov 25, 2021

WEB OF SCIENCETM
Citations

7
checked on Sep 30, 2021

Page view(s)

78
checked on Nov 18, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.