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|Title:||Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus||Authors:||Lee, K.-C.
|Issue Date:||1-Aug-2003||Citation:||Lee, K.-C., Lim, D., Wong, S.-M., Dokland, T. (2003-08-01). Purification, crystallization and X-ray analysis of Hibiscus chlorotic ringspot virus. Acta Crystallographica - Section D Biological Crystallography 59 (8) : 1481-1483. ScholarBank@NUS Repository. https://doi.org/10.1107/S0907444903011648||Abstract:||Hibiscus chlorotic ringspot virus (HCRSV), a Carmovirus, occurs worldwide and induces chlorotic ringspots on leaves, stunting and flower distortion in Hibiscus species, including kenaf. The HCRSV capsid has T = 3 icosahedral symmetry and contains 180 copies of the coat protein. A virus yield of 48-70 mg per 100 g of infected kenaf leaves was achieved with an improved purification scheme involving sucrose-cushion and sucrose density-gradient centrifugation. The virus was crystallized using PEG 8000 and 2,3-butanediol as co-precipitants. The crystals belonged to the cubic space group P23, with unit-cell parameter a = 392 Å, and diffracted X-rays to at least 4.5 Å resolution.||Source Title:||Acta Crystallographica - Section D Biological Crystallography||URI:||http://scholarbank.nus.edu.sg/handle/10635/101508||ISSN:||09074449||DOI:||10.1107/S0907444903011648|
|Appears in Collections:||Staff Publications|
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