Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0073725
Title: Profiling of Genes Related to Cross Protection and Competition for NbTOM1 by HLSV and TMV
Authors: Wen, Y.
Lim, G.X.-Y.
Wong, S.-M. 
Issue Date: 4-Sep-2013
Citation: Wen, Y., Lim, G.X.-Y., Wong, S.-M. (2013-09-04). Profiling of Genes Related to Cross Protection and Competition for NbTOM1 by HLSV and TMV. PLoS ONE 8 (9) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0073725
Abstract: Cross protection is the phenomenon through which a mild strain virus suppresses symptoms induced by a closely related severe strain virus in infected plants. Hibiscus latent Singapore virus (HLSV) and Tobacco mosaic virus (TMV) are species within the genus tobamovirus. HLSV can protect Nicotiana benthamiana against TMV-U1 strain, resulting in mild symptoms instead of severe systemic necrosis. The mechanism of cross protection between HLSV and TMV is unknown. In the past, some researchers suggest that the protecting virus strain might occupy virus-specific replication sites within a cell leaving no room for the challenge virus. Quantitative real-time RT-PCR was performed to detect viral RNA levels during cross protection. HLSV accumulation increased in cross protected plants compared with that of single HLSV infected plants, while TMV decreased in cross protected plants. This suggests that there is a competition for host factors between HLSV and TMV for replication. To investigate the mechanism under the cross protection between HLSV and TMV, microarray analysis was conducted to examine the transcriptional levels of global host genes during cross protection, using Tobacco Gene Expression Microarray, 4x44 k slides. The transcriptional level of some host genes corresponded to accumulation level of TMV. Some host genes were up-regulated only by HLSV. Tobamovirus multiplication gene 1 (TOM1), essential for tobamovirus multiplication, was involved in competition for replication by HLSV and TMV during cross protection. Both HLSV and TMV accumulation decreased when NbTOM1 was silenced. A large quantity of HLSV resulted in decreased TMV accumulation in HLSV+TMV (100:1) co-infection. These results indicate that host genes involved in the plant defense response and virus multiplication are up-regulated by challenge virus TMV but not by protecting virus HLSV during cross protection. © 2013 Wen et al.
Source Title: PLoS ONE
URI: http://scholarbank.nus.edu.sg/handle/10635/101465
ISSN: 19326203
DOI: 10.1371/journal.pone.0073725
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2013-profiling_genes_related_cross_protection-pub.pdf1.81 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

5
checked on Oct 21, 2021

WEB OF SCIENCETM
Citations

4
checked on Oct 21, 2021

Page view(s)

284
checked on Oct 14, 2021

Download(s)

6
checked on Oct 14, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.