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|Title:||Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus||Authors:||Cheng, A.
Hibiscus chlorotic ringspot virus
|Issue Date:||2009||Citation:||Cheng, A., Speir, J.A., Yuan, Y.A., Johnson, J.E., Wong, S.-M. (2009). Preliminary X-ray data analysis of crystalline hibiscus chlorotic ringspot virus. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 (6) : 589-593. ScholarBank@NUS Repository. https://doi.org/10.1107/S1744309109015760||Abstract:||Hibiscus chlorotic ringspot virus (HCRSV) is a positive-sense monopartite single-stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi-symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full-length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density-gradient centrifugation and anion-exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. The crystal belonged to space group R32, with unit-cell parameters a = b = 336.4, c = 798.5 Å. Packing considerations and rotation-function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real-space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high-resolution structure determination of HCRSV. © 2009 International Union of Crystallography. All rights reserved.||Source Title:||Acta Crystallographica Section F: Structural Biology and Crystallization Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/101448||ISSN:||17443091||DOI:||10.1107/S1744309109015760|
|Appears in Collections:||Staff Publications|
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