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|Title:||PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation||Authors:||Ruan, K.
|Issue Date:||14-Nov-2012||Citation:||Ruan, K., Ye, F., Li, C., Liou, Y.-C., Lin, S.-C., Lin, S.-Y. (2012-11-14). PLK1 Interacts and Phosphorylates Axin That Is Essential for Proper Centrosome Formation. PLoS ONE 7 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0049184||Abstract:||Abnormal amplification of centrosomes could lead to improper chromosome segregation and aneuploidy and is implicated in cancer development. Here, we demonstrate that Axin, a scaffolding protein in Wnt signaling, is phosphorylated by PLK1 during mitosis. Phosphorylation of Axin Ser-157 by PLK1 abolished Axin association with γ-tubulin, while substitution of Ser-157 with alanine exhibited sustained interaction with γ-tubulin. In addition, overexpression of Axin-S157A significantly increased the number of cells with multi-centrosomes. These results suggest that the phosphorylation status of Axin, mediated by PLK1, dynamically regulates its association with γ-tubulin and centrosome formation and segregation. © 2012 Ruan et al.||Source Title:||PLoS ONE||URI:||http://scholarbank.nus.edu.sg/handle/10635/101422||ISSN:||19326203||DOI:||10.1371/journal.pone.0049184|
|Appears in Collections:||Staff Publications|
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