Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bone.2013.01.026
DC Field | Value | |
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dc.title | Plasma membrane Ca2+ ATPase Atp2b1a regulates bone mineralization in zebrafish | |
dc.contributor.author | Go, W. | |
dc.contributor.author | Korzh, V. | |
dc.date.accessioned | 2014-10-27T08:36:55Z | |
dc.date.available | 2014-10-27T08:36:55Z | |
dc.date.issued | 2013-05 | |
dc.identifier.citation | Go, W., Korzh, V. (2013-05). Plasma membrane Ca2+ ATPase Atp2b1a regulates bone mineralization in zebrafish. Bone 54 (1) : 48-57. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bone.2013.01.026 | |
dc.identifier.issn | 87563282 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/101411 | |
dc.description.abstract | The zebrafish transgenic lines provide a possibility to observe the development of tissues and organs in real time. Using the reporter line for the zebrafish plasma membrane Ca2+ ATPase (SqET4), we detected its expression in the epithelium of pharyngeal teeth and analyzed its role in their calcification and that of cranial bones. atp2b1a's expression in the pharyngeal epithelium is faithfully recapitulated in the SqET4 transgenics by GFP expression. We showed by morpholino knockdown of Atp2b1a translations as well as chemical inhibition of Atp2b1a pump activity using carboxyeosin, that its activity is required to facilitate calcification of the developing pharyngeal teeth by the dental epithelium. Atp2b1a could be required during calcification of endochondral bones, where it acts at two levels: 1) by exporting Ca2+ from ameloblasts, it provides raw material for calcifying the pharyngeal teeth by adjacent odontoblasts; and 2) by regulating terminal differentiation of pharyngeal epithelial cells, including ameloblasts required for tissue hyper-mineralization. atp2b1a's expression in the pharyngeal epithelium is regulated by the homeodomain transcription factor dlx2b. © 2013 Elsevier Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bone.2013.01.026 | |
dc.source | Scopus | |
dc.subject | Ameloblasts | |
dc.subject | Calcification | |
dc.subject | Dlx2b | |
dc.subject | Pharyngeal epithelium | |
dc.subject | Plasma membrane Ca2+ pump | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1016/j.bone.2013.01.026 | |
dc.description.sourcetitle | Bone | |
dc.description.volume | 54 | |
dc.description.issue | 1 | |
dc.description.page | 48-57 | |
dc.description.coden | BONED | |
dc.identifier.isiut | 000316839800007 | |
Appears in Collections: | Staff Publications |
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