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Title: Pin1 facilitates the phosphorylation-dependent ubiquitination of SF-1 to regulate gonadotropin β-subunit gene transcription
Authors: Luo, Z.
Wijeweera, A.
Oh, Y.
Liou, Y.-C. 
Melamed, P. 
Issue Date: Feb-2010
Citation: Luo, Z., Wijeweera, A., Oh, Y., Liou, Y.-C., Melamed, P. (2010-02). Pin1 facilitates the phosphorylation-dependent ubiquitination of SF-1 to regulate gonadotropin β-subunit gene transcription. Molecular and Cellular Biology 30 (3) : 745-763. ScholarBank@NUS Repository.
Abstract: Pin1 is a peptidyl-prolyl cis-trans isomerase which catalyzes the isomerization of phosphorylated Ser/Thr-Pro peptide bonds. Pin1 knockout mice have marked abnormalities in their reproductive development and function. However, the molecular mechanisms underlying their reproductive defects are poorly understood. Herein, we demonstrate that Pin1 is required for both basal and GnRH-induced gonadotropin β-subunit gene transcription, through interactions with the transcription factors SF-1, Pitx1, and Egr-1. Pin1 activates transcription of the gonadotropin β-subunit genes synergistically with these transcription factors, either by modulating their stability or by increasing their protein-protein interactions. Notably, we provide evidence that Pin1 is required for the Ser203 phosphorylation-dependent ubiquitination of SF-1, which facilitates SF-1-Pitx1 interactions and therefore results in an enhancement of SF-1 transcriptional activity. Furthermore, we demonstrate that in gonadotrope cells, sufficient levels of activated Pin1 are maintained through transcriptional and posttranslational regulation by GnRH-induced signaling cascades. Our results suggest that Pin1 functions as a novel player in GnRH-induced signal pathways and is involved in gonadotropin β-subunit gene transcription by modulating the activity of various specific transcription factors. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Source Title: Molecular and Cellular Biology
ISSN: 02707306
DOI: 10.1128/MCB.00807-09
Appears in Collections:Staff Publications

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