Please use this identifier to cite or link to this item:
Title: Osterix-mCherry transgenic medaka for in vivo imaging of bone formation
Authors: Renn, J. 
Winkler, C. 
Keywords: Bone formation
Life imaging
Transgenic fish
Issue Date: Jan-2009
Citation: Renn, J., Winkler, C. (2009-01). Osterix-mCherry transgenic medaka for in vivo imaging of bone formation. Developmental Dynamics 238 (1) : 241-248. ScholarBank@NUS Repository.
Abstract: Intramembranous and chondral bone formation by osteoblasts is found in all vertebrates. The genetic network controlling osteoblast differentiation is highly conserved and regulated by a small number of key factors, including the zinc-finger transcription factor Osterix. Expression analysis of osterix in the teleost model medaka revealed a highly restricted expression in skeletal regions. For in vivo imaging, we generated transgenic medaka expressing mCherry under control of the osterix promoter. We show that the transgene becomes expressed in early osteoblasts, which have not yet mineralized bone matrix, and remains high in matured and mineralizing osteoblasts. Life imaging of transgenic larvae provided insight into the appearance and behavior of early osteoblasts during development of the teleost cranium, vertebrae, and caudal fin. In summary, osterix-mCherry transgenic medaka enable us to analyze osteoblasts during different maturation phases in vivo and represent a unique tool to study osteoblast behavior in vertebrate embryos and adults. © 2008 Wiley-Liss, Inc.
Source Title: Developmental Dynamics
ISSN: 10588388
DOI: 10.1002/dvdy.21836
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.