Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.gene.2005.12.002
Title: Ohanin, a novel protein from king cobra venom: Its cDNA and genomic organization
Authors: Pung, Y.F.
Kumar, S.V.
Rajagopalan, N.
Fry, B.G. 
Kumar, P.P. 
Kini, R.M. 
Keywords: Alternative splicing
B30.2-like domain
PRY-SPRY domains
Vespryns
Issue Date: 26-Apr-2006
Citation: Pung, Y.F., Kumar, S.V., Rajagopalan, N., Fry, B.G., Kumar, P.P., Kini, R.M. (2006-04-26). Ohanin, a novel protein from king cobra venom: Its cDNA and genomic organization. Gene 371 (2) : 246-256. ScholarBank@NUS Repository. https://doi.org/10.1016/j.gene.2005.12.002
Abstract: Ohanin, from king cobra venom, is a novel protein which induces hypolocomotion and hyperalgesia in mice [Pung, Y.F., Wong, P.T.H., Kumar, P.P., Hodgson W.C., Kini, R.M., 2005. Ohanin, a novel protein from king cobra venom induces hypolocomotion and hyperalgesia in mice. J. Biol. Chem. 280, 13137-13147.]. It is weakly similar to PRY-SPRY domains (B30.2-like domain). Here we report the complete cDNA and genomic organization of ohanin. Interestingly, cDNA sequence does not show significant sequence similarity to any known sequences, including those of B30.2-like domain-containing proteins. Its full-length cDNA sequence of 1558 bp encodes for prepro-ohanin with a propeptide segment at the C-terminal. Ohanin is the first member of a new subfamily of proteins containing B30.2-like domain with short N-terminal segment. We named this subfamily as vespryns. There are two mRNA subtypes differing in their 5′-untranslated regions. Southern hybridization study shows that ohanin is encoded by a single gene. Its genomic sequence is 7086 bp with five exons and four introns, and the two types of mRNAs are generated by alternative splicing of exon 2. Our results indicate that ohanin and vespryns may have evolved from the same ancestral gene as B30.2 domain. © 2006 Elsevier B.V. All rights reserved.
Source Title: Gene
URI: http://scholarbank.nus.edu.sg/handle/10635/101257
ISSN: 03781119
DOI: 10.1016/j.gene.2005.12.002
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