Please use this identifier to cite or link to this item:
Title: Ohanin, a novel protein from king cobra venom: Its cDNA and genomic organization
Authors: Pung, Y.F.
Kumar, S.V.
Rajagopalan, N.
Fry, B.G. 
Kumar, P.P. 
Kini, R.M. 
Keywords: Alternative splicing
B30.2-like domain
PRY-SPRY domains
Issue Date: 26-Apr-2006
Citation: Pung, Y.F., Kumar, S.V., Rajagopalan, N., Fry, B.G., Kumar, P.P., Kini, R.M. (2006-04-26). Ohanin, a novel protein from king cobra venom: Its cDNA and genomic organization. Gene 371 (2) : 246-256. ScholarBank@NUS Repository.
Abstract: Ohanin, from king cobra venom, is a novel protein which induces hypolocomotion and hyperalgesia in mice [Pung, Y.F., Wong, P.T.H., Kumar, P.P., Hodgson W.C., Kini, R.M., 2005. Ohanin, a novel protein from king cobra venom induces hypolocomotion and hyperalgesia in mice. J. Biol. Chem. 280, 13137-13147.]. It is weakly similar to PRY-SPRY domains (B30.2-like domain). Here we report the complete cDNA and genomic organization of ohanin. Interestingly, cDNA sequence does not show significant sequence similarity to any known sequences, including those of B30.2-like domain-containing proteins. Its full-length cDNA sequence of 1558 bp encodes for prepro-ohanin with a propeptide segment at the C-terminal. Ohanin is the first member of a new subfamily of proteins containing B30.2-like domain with short N-terminal segment. We named this subfamily as vespryns. There are two mRNA subtypes differing in their 5′-untranslated regions. Southern hybridization study shows that ohanin is encoded by a single gene. Its genomic sequence is 7086 bp with five exons and four introns, and the two types of mRNAs are generated by alternative splicing of exon 2. Our results indicate that ohanin and vespryns may have evolved from the same ancestral gene as B30.2 domain. © 2006 Elsevier B.V. All rights reserved.
Source Title: Gene
ISSN: 03781119
DOI: 10.1016/j.gene.2005.12.002
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Apr 15, 2021


checked on Apr 15, 2021

Page view(s)

checked on Apr 11, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.