Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M604409200
Title: Neuronal PAS domain protein 1 is a transcriptional repressor and requires arylhydrocarbon nuclear translocator for its nuclear localization
Authors: Teh, C.H.L.
Lam, K.K.Y.
Loh, C.C.
Loo, J.M.
Yan, T.
Lim, T.M. 
Issue Date: 10-Nov-2006
Citation: Teh, C.H.L., Lam, K.K.Y., Loh, C.C., Loo, J.M., Yan, T., Lim, T.M. (2006-11-10). Neuronal PAS domain protein 1 is a transcriptional repressor and requires arylhydrocarbon nuclear translocator for its nuclear localization. Journal of Biological Chemistry 281 (45) : 34617-34629. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M604409200
Abstract: Neuronal PAS domain protein 1 (NPAS1), a basic helix-loop-helix-PAS transcription factor expressed in the central nervous system, has been suggested to be involved in neuronal differentiation. However, relatively little is known about the molecular mechanism underlying the role of NPAS1 during development. In this study we set out to characterize the different domains within NPAS1. We showed that the nuclear localization of NPAS1 is dependent on the presence of ARNT. In addition, the transcriptional potential of ARNT is not required for this localization. In the absence of ARNT, NPAS1 is excluded from the nucleus, and this exclusion is due to the presence of a nuclear export signal within the N terminus of NPAS1. The interaction between NPAS1 and ARNT is via their N termini. We found no transactivation domain within NPAS1; instead, we mapped out at least three repression domains within NPAS1, suggesting that NPAS1 acts as a repressor. Furthermore, our experiments showed that NPAS1 is able to repress the transactivation functions of ARNT and ARNT2. We suggest that NPAS1 is guided into the nucleus by ARNT via the ARNT nuclear localization signal, and NPAS1 can override the activation function of adjacent transcription factors, providing a mechanism by which NPAS1 may inhibit transcription. © 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/101196
ISSN: 00219258
DOI: 10.1074/jbc.M604409200
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

17
checked on Oct 20, 2021

WEB OF SCIENCETM
Citations

17
checked on Oct 20, 2021

Page view(s)

105
checked on Oct 14, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.