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Title: Morphologic transformation of the thymus in developing zebrafish
Authors: Lam, S.H. 
Chua, H.L.
Gong, Z. 
Wen, Z. 
Lam, T.J. 
Sin, Y.M. 
Keywords: Cortex-medullary regionalization
Morphologic transformation
Recombination activating gene-1
T-cell receptor
Zebrafish development
Issue Date: Sep-2002
Citation: Lam, S.H., Chua, H.L., Gong, Z., Wen, Z., Lam, T.J., Sin, Y.M. (2002-09). Morphologic transformation of the thymus in developing zebrafish. Developmental Dynamics 225 (1) : 87-94. ScholarBank@NUS Repository.
Abstract: The morphologic transformation of the developing zebrafish thymus from 1 week postfertilization (wpf) to 15 wpf is described. The thymus overall morphology changed from a small pouch-like shape at 1 wpf to a conical shape between 2 and 3 wpf before acquiring a more complex shape from 4 wpf onward. Rapid growth rate along the lateral axis at the region near the pharyngeal epithelium occurred between 1 and 2 wpf, whereas rapid growth rate along the dorsal-ventral axis occurred between 3 and 6 wpf. Expansion of thymocyte population beginning from 1 wpf became more evident by 2 to 3 wpf, as indicated by the apparent increase of different sizes of lymphocytes, recombination activating gene-1 (rag-1), and T-cell antigen receptor alpha chain constant region (TCRAC) -positive cells. Tissue section in situ hybridization (ISH) analysis with rag-1 probe reveals that cortex-medullary regionalization has begun between 1 and 2 wpf as rag-1 expression clearly demarcated the cortex, whereas the medulla was rag-1 negative. The presence of TCRAC-positive cells in the medulla by 2-3 wpf, suggests that the thymic selection processes had begun. The zebrafish thymus is morphologically mature by 3 wpf. Early signs of thymic involution were observed in zebrafish aged 15 wpf. © 2002 Wiley-Liss, Inc.
Source Title: Developmental Dynamics
ISSN: 10588388
DOI: 10.1002/dvdy.10127
Appears in Collections:Staff Publications

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