Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1005756822370
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dc.titleMorphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium
dc.contributor.authorKwa, S.-H.
dc.contributor.authorWee, Y.-C.
dc.contributor.authorLim, T.-M.
dc.contributor.authorKumar, P.P.
dc.date.accessioned2014-10-27T08:34:08Z
dc.date.available2014-10-27T08:34:08Z
dc.date.issued1997
dc.identifier.citationKwa, S.-H., Wee, Y.-C., Lim, T.-M., Kumar, P.P. (1997). Morphogenetic plasticity of callus reinitiated from cell suspension cultures of the fern Platycerium coronarium. Plant Cell, Tissue and Organ Culture 48 (1) : 37-44. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1005756822370
dc.identifier.issn01676857
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101156
dc.description.abstractCell suspension cultures were initiated from gametophyte-derived callus of the fern Platycerium coronarium. Two distinct types of callus masses, distinguished by their colouration, were obtained when the cells from suspension culture were plated on semisolid Murashige and Skoog (MS) medium containing 10 μM kinetin. The two types of callus masses had distinct morphogenetic capacities despite their common origin. Morphogenesis into either gametophytes or sporophytes occurred when these callus masses were cultured on phytohormone-free MS medium depending on the type of callus used. The dark-green gametophytic callus showed a faster rate of growth and morphogenesis as compared to the pale-green sporophytic callus. Total chlorophyll content and auto fluorescence and size of chloroplasts of the sporophytic callus and cell suspension cultures were lower than that of the gametophytic callus. Observations from con focal laser scanning microscopy were in agreement with the physiological parameters measured. The availability of cell cultures of the same ploidy level, but with two distinct pathways of development will be useful for comparative studies of developmental plasticity.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1023/A:1005756822370
dc.sourceScopus
dc.subjectCell suspension cultures
dc.subjectConfocal laser scanning microscopy
dc.subjectGametophyte
dc.subjectMorphogenetic plasticity
dc.subjectPlatycerium coronarium
dc.subjectSporophyte
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1023/A:1005756822370
dc.description.sourcetitlePlant Cell, Tissue and Organ Culture
dc.description.volume48
dc.description.issue1
dc.description.page37-44
dc.description.codenPTCED
dc.identifier.isiutA1997XF02900006
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