Please use this identifier to cite or link to this item:
https://doi.org/10.1021/ja413031h
DC Field | Value | |
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dc.title | Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions | |
dc.contributor.author | Goh, W.L. | |
dc.contributor.author | Lee, M.Y. | |
dc.contributor.author | Joseph, T.L. | |
dc.contributor.author | Quah, S.T. | |
dc.contributor.author | Brown, C.J. | |
dc.contributor.author | Verma, C. | |
dc.contributor.author | Brenner, S. | |
dc.contributor.author | Ghadessy, F.J. | |
dc.contributor.author | Teo, Y.N. | |
dc.date.accessioned | 2014-10-27T08:34:04Z | |
dc.date.available | 2014-10-27T08:34:04Z | |
dc.date.issued | 2014-04-30 | |
dc.identifier.citation | Goh, W.L., Lee, M.Y., Joseph, T.L., Quah, S.T., Brown, C.J., Verma, C., Brenner, S., Ghadessy, F.J., Teo, Y.N. (2014-04-30). Molecular rotors as conditionally fluorescent labels for rapid detection of biomolecular interactions. Journal of the American Chemical Society 136 (17) : 6159-6162. ScholarBank@NUS Repository. https://doi.org/10.1021/ja413031h | |
dc.identifier.issn | 15205126 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/101150 | |
dc.description.abstract | We demonstrate the use of fluorescent molecular rotors as probes for detecting biomolecular interactions, specifically peptide-protein interactions. Molecular rotors undergo twisted intramolecular charge transfer upon irradiation, relax via the nonradiative torsional relaxation pathway, and have been typically used as viscosity probes. Their utility as a tool for detecting specific biomolecular interactions has not been explored. Using the well characterized p53-Mdm2 interaction as a model system, we designed a 9-(2-carboxy-2-cyanovinyl) julolidine-based p53 peptide reporter, JP1-R, which fluoresces conditionally only upon Mdm2 binding. The reporter was used in a rapid, homogeneous assay to screen a fragment library for antagonists of the p53-Mdm2 interaction, and several inhibitors were identified. Subsequent validation of these hits using established secondary assays suggests increased sensitivity afforded by JP1-R. The fluorescence of molecular rotors contingent upon target binding makes them a versatile tool for detecting specific biomolecular interactions. © 2014 American Chemical Society. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/ja413031h | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1021/ja413031h | |
dc.description.sourcetitle | Journal of the American Chemical Society | |
dc.description.volume | 136 | |
dc.description.issue | 17 | |
dc.description.page | 6159-6162 | |
dc.description.coden | JACSA | |
dc.identifier.isiut | 000335369200003 | |
Appears in Collections: | Staff Publications |
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