Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.01233-10
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dc.titleModulation of pseudomonas aeruginosa biofilm dispersal by a cyclic-di-gmp phosphodiesterase with a putative hypoxia-sensing domain
dc.contributor.authorAn, S.
dc.contributor.authorWu, J.
dc.contributor.authorZhang, L.-H.
dc.date.accessioned2014-10-27T08:33:42Z
dc.date.available2014-10-27T08:33:42Z
dc.date.issued2010-12
dc.identifier.citationAn, S., Wu, J., Zhang, L.-H. (2010-12). Modulation of pseudomonas aeruginosa biofilm dispersal by a cyclic-di-gmp phosphodiesterase with a putative hypoxia-sensing domain. Applied and Environmental Microbiology 76 (24) : 8160-8173. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.01233-10
dc.identifier.issn00992240
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101115
dc.description.abstractPseudomonas aeruginosa encodes many enzymes that are potentially associated with the synthesis or degradation of the widely conserved second messenger cyclic-di-GMP (c-di-GMP). In this study, we show that mutation of rbdA, which encodes a fusion protein consisting of PAS-PAC-GGDEF-EAL multidomains, results in decreased biofilm dispersal. RbdA contains a highly conserved GGDEF domain and EAL domain, which are involved in the synthesis and degradation of c-di-GMP, respectively. However, in vivo and in vitro analyses show that the full-length RbdA protein only displays phosphodiesterase activity, causing c-di-GMP degradation. Further analysis reveals that the GGDEF domain of RbdA plays a role in activating the phosphodiesterase activity of the EAL domain in the presence of GTP. Moreover, we show that deletion of the PAS domain or substitution of the key residues implicated in sensing low-oxygen stress abrogates the functionality of RbdA. Subsequent study showed that RbdA is involved in positive regulation of bacterial motility and production of rhamnolipids, which are associated with biofilm dispersal, and in negative regulation of production of exopolysaccharides, which are required for biofilm formation. These data indicate that the c-di-GMP-degrading regulatory protein RbdA promotes biofilm dispersal through its two-pronged effects on biofilm development, i.e., downregulating biofilm formation and upregulating production of the factors associated with biofilm dispersal. © 2010, American Society for Microbiology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/AEM.01233-10
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1128/AEM.01233-10
dc.description.sourcetitleApplied and Environmental Microbiology
dc.description.volume76
dc.description.issue24
dc.description.page8160-8173
dc.description.codenAEMID
dc.identifier.isiut000284882800033
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