Please use this identifier to cite or link to this item:
Title: Modulation of bacterial type III secretion system by a spermidine transporter dependent signaling pathway
Authors: Zhou, L.
Wang, J.
Zhang, L.-H. 
Issue Date: 12-Dec-2007
Citation: Zhou, L., Wang, J., Zhang, L.-H. (2007-12-12). Modulation of bacterial type III secretion system by a spermidine transporter dependent signaling pathway. PLoS ONE 2 (12) : -. ScholarBank@NUS Repository.
Abstract: Background. Many gram-negative bacterial pathogens employ Type III secretion systems (T3SS) to inject effector proteins into host cells in infectious processes. Methodology/Principal Findings. By screening a transposon mutant library of P. aeruginosa, we found that mutation of spuDEFGH, which encode a major spermidine uptake system, abolished the expression of the exsCEBA operon that codes for key T3SS regulators under inducing conditions (low calcium). Whole genome microarray analysis revealed that inactivation of the spermidine uptake system significantly decreased the transcriptional expression of most, if not all, T3SS genes. Consistently, the spermidine uptake mutants showed decreased expression of the T3SS genes in responding to host cell extract and attenuated cytotoxicity. Furthermore, exogenous addition of spermidine to the wild type strain PAO1 enhanced the expression of exsCEBA and also the effector protein genes. Conclusion/Significance. Cumulatively, these data have depicted a novel spermidine transporter-dependent signaling pathway, which appears to play an essential role in modulation of T3SS expression in P. aeruginosa. © 2007 Zhou et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0001291
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
10_1371_journal_pone_0001291.pdf558.5 kBAdobe PDF




checked on Jun 29, 2022

Page view(s)

checked on Jun 23, 2022


checked on Jun 23, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.