Please use this identifier to cite or link to this item: https://doi.org/10.1002/dvdy.20444
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dc.titleMicroarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes
dc.contributor.authorQian, F.
dc.contributor.authorZhen, F.
dc.contributor.authorOng, C.
dc.contributor.authorJin, S.-W.
dc.contributor.authorSoo, H.M.
dc.contributor.authorStainier, D.Y.R.
dc.contributor.authorLin, S.
dc.contributor.authorPeng, J.
dc.contributor.authorWen, Z.
dc.date.accessioned2014-10-27T08:33:24Z
dc.date.available2014-10-27T08:33:24Z
dc.date.issued2005-07
dc.identifier.citationQian, F., Zhen, F., Ong, C., Jin, S.-W., Soo, H.M., Stainier, D.Y.R., Lin, S., Peng, J., Wen, Z. (2005-07). Microarray analysis of zebrafish cloche mutant using amplified cDNA and identification of potential downstream target genes. Developmental Dynamics 233 (3) : 1163-1172. ScholarBank@NUS Repository. https://doi.org/10.1002/dvdy.20444
dc.identifier.issn10588388
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101089
dc.description.abstractZebrafish is an excellent model organism for studying vertebrate development and human disease. With the availability of increased numbers of zebrafish mutants and microarray chips, gene expression profiling has become a powerful tool for identification of downstream target genes perturbed by a specific mutation. One of the obstacles often encountered, however, is to isolate large numbers of zebrafish mutant embryos that are indistinguishable in morphology from the wild-type siblings for microarray analysis. Here, we report a method using amplified cDNA derived from five embryos for gene expression profiling of the 18-somite zebrafish cloche (clo) mutant, in which development of hematopoietic and endothelial lineages is severely impaired. In total, 31 differentially expressed target genes are identified, of which 13 have not been reported previously. We further determine that of these 13 new targets, 8 genes, including coproporphyrinogen oxidase (cpo), carbonic anhydrase (cahz), claudin g (cldn g), zinc-finger-like gene 2 (znfl2), neutrophil cytosol factor 1 (ncf1), matrix metalloproteinase 13 (mmp13), dual specificity phosphatase 5 (dusp5), and a novel gene referred as zebrafish vessel-specific gene 1 (zvsg1) are predominantly expressed in hematopoietic and endothelial cells. Comparative analysis demonstrates that this method is comparable and complementary to that of the conventional approach using unamplified sample. Our study provides valuable information for studying hematopoiesis and vessel formation. The method described here offers a powerful tool for gene expression profiling of zebrafish mutants in general. © 2005 Wiley-Liss, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/dvdy.20444
dc.sourceScopus
dc.subjectcloche
dc.subjectHematopoiesis
dc.subjectMicroarray
dc.subjectZebrafish
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1002/dvdy.20444
dc.description.sourcetitleDevelopmental Dynamics
dc.description.volume233
dc.description.issue3
dc.description.page1163-1172
dc.description.codenDEDYE
dc.identifier.isiut000229892100051
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