Please use this identifier to cite or link to this item: https://doi.org/10.1023/B:JNMR.0000013826.82936.0e
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dc.titleMeasurement of dipolar cross-correlation in methylene groups in uniformly 13C-, 15N-labeled proteins
dc.contributor.authorZheng, Y.
dc.contributor.authorYang, D.
dc.date.accessioned2014-10-27T08:33:10Z
dc.date.available2014-10-27T08:33:10Z
dc.date.issued2004-02
dc.identifier.citationZheng, Y., Yang, D. (2004-02). Measurement of dipolar cross-correlation in methylene groups in uniformly 13C-, 15N-labeled proteins. Journal of Biomolecular NMR 28 (2) : 103-116. ScholarBank@NUS Repository. https://doi.org/10.1023/B:JNMR.0000013826.82936.0e
dc.identifier.issn09252738
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101067
dc.description.abstractA CC(CO)NH TOCSY-based 3D pulse scheme is presented for measuring 1H-13C dipole-dipole cross-correlated relaxation at CH2 positions in uniformly 13C-, 15N-labeled proteins. Simulations based on magnetization evolution under relaxation and scalar coupling interactions show that cross-correlation rates between 1H-13C dipoles in CH2 groups can be simply obtained from the intensities of 13C triplets. The normalized cross-correlation relaxation rates are related to cross-correlation order parameters for macromolecules undergoing isotropic motion, which reflect the degrees of spatial restriction of CH2 groups. The study on human intestinal fatty acid binding protein (131 residues) in the presence of oleic acid demonstrates that side chain dynamics at most CH2 positions can be characterized for proteins less than 15 kDa in size, with the proposed TOCSY-based approach.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1023/B:JNMR.0000013826.82936.0e
dc.sourceScopus
dc.subjectCross-correlated relaxation
dc.subjectFatty acid binding protein
dc.subjectProtein side chain dynamics
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1023/B:JNMR.0000013826.82936.0e
dc.description.sourcetitleJournal of Biomolecular NMR
dc.description.volume28
dc.description.issue2
dc.description.page103-116
dc.description.codenJBNME
dc.identifier.isiut000189177600001
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