Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0080221
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dc.titleMapping the structural and dynamical features of multiple p53 DNA binding domains: Insights into loop 1 intrinsic dynamics
dc.contributor.authorLukman, S.
dc.contributor.authorLane, D.P.
dc.contributor.authorVerma, C.S.
dc.date.accessioned2014-10-27T08:33:05Z
dc.date.available2014-10-27T08:33:05Z
dc.date.issued2013-11-12
dc.identifier.citationLukman, S., Lane, D.P., Verma, C.S. (2013-11-12). Mapping the structural and dynamical features of multiple p53 DNA binding domains: Insights into loop 1 intrinsic dynamics. PLoS ONE 8 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0080221
dc.identifier.issn19326203
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101058
dc.description.abstractThe transcription factor p53 regulates cellular integrity in response to stress. p53 is mutated in more than half of cancerous cells, with a majority of the mutations localized to the DNA binding domain (DBD). In order to map the structural and dynamical features of the DBD, we carried out multiple copy molecular dynamics simulations (totaling 0.8 μs). Simulations show the loop 1 to be the most dynamic element among the DNA-contacting loops (loops 1-3). Loop 1 occupies two major conformational states: extended and recessed; the former but not the latter displays correlations in atomic fluctuations with those of loop 2 (∼24 Å apart). Since loop 1 binds to the major groove whereas loop 2 binds to the minor groove of DNA, our results begin to provide some insight into the possible mechanism underpinning the cooperative nature of DBD binding to DNA. We propose (1) a novel mechanism underlying the dynamics of loop 1 and the possible tread-milling of p53 on DNA and (2) possible mutations on loop 1 residues to restore the transcriptional activity of an oncogenic mutation at a distant site. © 2013 Lukman et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0080221
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pone.0080221
dc.description.sourcetitlePLoS ONE
dc.description.volume8
dc.description.issue11
dc.description.page-
dc.description.codenPOLNC
dc.identifier.isiut000327252100135
dc.published.statePublished
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