Please use this identifier to cite or link to this item: https://doi.org/10.1096/fj.11-202663
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dc.titleMacrophage-stimulating protein and calcium homeostasis in zebrafish
dc.contributor.authorHuitema, L.F.A.
dc.contributor.authorRenn, J.
dc.contributor.authorLogister, I.
dc.contributor.authorGray, J.K.
dc.contributor.authorWaltz, S.E.
dc.contributor.authorFlik, G.
dc.contributor.authorSchulte-Merker, S.
dc.date.accessioned2014-10-27T08:32:57Z
dc.date.available2014-10-27T08:32:57Z
dc.date.issued2012-10
dc.identifier.citationHuitema, L.F.A., Renn, J., Logister, I., Gray, J.K., Waltz, S.E., Flik, G., Schulte-Merker, S. (2012-10). Macrophage-stimulating protein and calcium homeostasis in zebrafish. FASEB Journal 26 (10) : 4092-4101. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.11-202663
dc.identifier.issn15306860
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101047
dc.description.abstractTo systematically identify novel gene functions essential for osteogenesis and skeletal mineralization, we performed a forward genetic mutagenesis screen in zebrafish and isolated a mutant that showed delayed skeletal mineralization. Analysis of the mutant phenotype in an osterix:nuclear-GFP transgenic background demonstrated that mutants contain osterix-expressing osteoblasts comparable to wild-type embryos. Positional cloning revealed a premature stop mutation in the macrophage-stimulating protein (msp) gene, predicted to result in a biologically inactive protein. Analysis of the embryonic expression pattern for the receptor for Msp, Ron, shows specific expression in the corpuscles of Stannius, a teleost-specific organ that produces stanniocalcin, a pivotal hormone in fish calcium homeostasis. Knockdown of Ron resulted in identical phenotypes as observed in msp mutants. Msp mutant embryos could be rescued by excess calcium. Consistent with a role for Msp/Ron in calcium homeostasis, calcium-regulating factors, such as pth1, pth2, stc1l, and trpv5/6 were significantly affected in msp mutant larvae. While Msp and Ron have previously been shown to play a critical role in a wide variety of biological processes, we introduce here the Msp/Ron signaling axis as a previously unappreciated player in calcium homeostasis and embryonic skeletal mineralization. © FASEB.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1096/fj.11-202663
dc.sourceScopus
dc.subjectBone
dc.subjectMineralization
dc.subjectMsp
dc.subjectOsteogenesis
dc.subjectRon
dc.subjectStanniocalcin
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1096/fj.11-202663
dc.description.sourcetitleFASEB Journal
dc.description.volume26
dc.description.issue10
dc.description.page4092-4101
dc.description.codenFAJOE
dc.identifier.isiut000309704000016
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