Please use this identifier to cite or link to this item:
https://doi.org/10.1096/fj.11-202663
DC Field | Value | |
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dc.title | Macrophage-stimulating protein and calcium homeostasis in zebrafish | |
dc.contributor.author | Huitema, L.F.A. | |
dc.contributor.author | Renn, J. | |
dc.contributor.author | Logister, I. | |
dc.contributor.author | Gray, J.K. | |
dc.contributor.author | Waltz, S.E. | |
dc.contributor.author | Flik, G. | |
dc.contributor.author | Schulte-Merker, S. | |
dc.date.accessioned | 2014-10-27T08:32:57Z | |
dc.date.available | 2014-10-27T08:32:57Z | |
dc.date.issued | 2012-10 | |
dc.identifier.citation | Huitema, L.F.A., Renn, J., Logister, I., Gray, J.K., Waltz, S.E., Flik, G., Schulte-Merker, S. (2012-10). Macrophage-stimulating protein and calcium homeostasis in zebrafish. FASEB Journal 26 (10) : 4092-4101. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.11-202663 | |
dc.identifier.issn | 15306860 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/101047 | |
dc.description.abstract | To systematically identify novel gene functions essential for osteogenesis and skeletal mineralization, we performed a forward genetic mutagenesis screen in zebrafish and isolated a mutant that showed delayed skeletal mineralization. Analysis of the mutant phenotype in an osterix:nuclear-GFP transgenic background demonstrated that mutants contain osterix-expressing osteoblasts comparable to wild-type embryos. Positional cloning revealed a premature stop mutation in the macrophage-stimulating protein (msp) gene, predicted to result in a biologically inactive protein. Analysis of the embryonic expression pattern for the receptor for Msp, Ron, shows specific expression in the corpuscles of Stannius, a teleost-specific organ that produces stanniocalcin, a pivotal hormone in fish calcium homeostasis. Knockdown of Ron resulted in identical phenotypes as observed in msp mutants. Msp mutant embryos could be rescued by excess calcium. Consistent with a role for Msp/Ron in calcium homeostasis, calcium-regulating factors, such as pth1, pth2, stc1l, and trpv5/6 were significantly affected in msp mutant larvae. While Msp and Ron have previously been shown to play a critical role in a wide variety of biological processes, we introduce here the Msp/Ron signaling axis as a previously unappreciated player in calcium homeostasis and embryonic skeletal mineralization. © FASEB. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1096/fj.11-202663 | |
dc.source | Scopus | |
dc.subject | Bone | |
dc.subject | Mineralization | |
dc.subject | Msp | |
dc.subject | Osteogenesis | |
dc.subject | Ron | |
dc.subject | Stanniocalcin | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1096/fj.11-202663 | |
dc.description.sourcetitle | FASEB Journal | |
dc.description.volume | 26 | |
dc.description.issue | 10 | |
dc.description.page | 4092-4101 | |
dc.description.coden | FAJOE | |
dc.identifier.isiut | 000309704000016 | |
Appears in Collections: | Staff Publications |
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