Please use this identifier to cite or link to this item:
|Title:||Macrophage-stimulating protein and calcium homeostasis in zebrafish||Authors:||Huitema, L.F.A.
|Issue Date:||Oct-2012||Citation:||Huitema, L.F.A., Renn, J., Logister, I., Gray, J.K., Waltz, S.E., Flik, G., Schulte-Merker, S. (2012-10). Macrophage-stimulating protein and calcium homeostasis in zebrafish. FASEB Journal 26 (10) : 4092-4101. ScholarBank@NUS Repository. https://doi.org/10.1096/fj.11-202663||Abstract:||To systematically identify novel gene functions essential for osteogenesis and skeletal mineralization, we performed a forward genetic mutagenesis screen in zebrafish and isolated a mutant that showed delayed skeletal mineralization. Analysis of the mutant phenotype in an osterix:nuclear-GFP transgenic background demonstrated that mutants contain osterix-expressing osteoblasts comparable to wild-type embryos. Positional cloning revealed a premature stop mutation in the macrophage-stimulating protein (msp) gene, predicted to result in a biologically inactive protein. Analysis of the embryonic expression pattern for the receptor for Msp, Ron, shows specific expression in the corpuscles of Stannius, a teleost-specific organ that produces stanniocalcin, a pivotal hormone in fish calcium homeostasis. Knockdown of Ron resulted in identical phenotypes as observed in msp mutants. Msp mutant embryos could be rescued by excess calcium. Consistent with a role for Msp/Ron in calcium homeostasis, calcium-regulating factors, such as pth1, pth2, stc1l, and trpv5/6 were significantly affected in msp mutant larvae. While Msp and Ron have previously been shown to play a critical role in a wide variety of biological processes, we introduce here the Msp/Ron signaling axis as a previously unappreciated player in calcium homeostasis and embryonic skeletal mineralization. © FASEB.||Source Title:||FASEB Journal||URI:||http://scholarbank.nus.edu.sg/handle/10635/101047||ISSN:||15306860||DOI:||10.1096/fj.11-202663|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 14, 2019
WEB OF SCIENCETM
checked on Aug 14, 2019
checked on Aug 17, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.