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Title: Lysozyme transgenic Atlantic salmon (Salmo salar L.)
Authors: Fletcher, G.L.
Hobbs, R.S.
Evans, R.P.
Shears, M.A.
Hahn, A.L.
Hew, C.L. 
Keywords: Antifreeze protein gene promoter
Disease resistance
Rainbow trout lysozyme sequence
Transgene expression
Issue Date: Feb-2011
Citation: Fletcher, G.L., Hobbs, R.S., Evans, R.P., Shears, M.A., Hahn, A.L., Hew, C.L. (2011-02). Lysozyme transgenic Atlantic salmon (Salmo salar L.). Aquaculture Research 42 (3) : 427-440. ScholarBank@NUS Repository.
Abstract: Disease outbreaks are a major constraint to salmon aquaculture worldwide. Therefore, there is considerable interest in finding means to alleviate this problem by enhancing their innate immune system. Because lysozyme is an important component of this system, we generated a line of transgenic Atlantic salmon using a gene construct consisting of a rainbow trout lysozyme gene under the control of the ocean pout antifreeze protein gene promoter op5a (opAFP-rtLys). The results show that the transgene exhibited Mendelian inheritance at both the F1 and the F2 generations and demonstrated that it had integrated into a single chromosome in the genetic founder. Sequence analysis of F2 generation salmon revealed the presence of a complete copy of an intact lysozyme transgene integrant that was identical in sequence to that of the construct. The computationally translated peptide sequence of the lysozyme coding region differed by four amino acids from those present in GenBank (accession nos CAA42084; AAG34564). Reverse transcriptase-polymerase chain reaction analysis demonstrated the expression of the transgene in most body tissues. However, Northern analysis revealed that visible levels of mRNA were only evident in the spleen, skin and kidney. The lysozyme lytic activity of kidney tissue extracts from F2 transgenic salmon was 40% greater than it was in non-transgenic siblings. © 2010 Blackwell Publishing Ltd.
Source Title: Aquaculture Research
ISSN: 1355557X
DOI: 10.1111/j.1365-2109.2010.02637.x
Appears in Collections:Staff Publications

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