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Title: Loss of function of def selectively up-regulates Δ113p53 expression to arrest expansion growth of digestive organs in zebrafish
Authors: Chen, J.
Ruan, H.
Sok, M.N.
Gao, C.
Hui, M.S.
Wu, W.
Zhang, Z.
Wen, Z.
Lane, D.P.
Peng, J. 
Keywords: Def (digestive-organ expansion factor)
Endoderm organogenesis
Issue Date: 1-Dec-2005
Citation: Chen, J., Ruan, H., Sok, M.N., Gao, C., Hui, M.S., Wu, W., Zhang, Z., Wen, Z., Lane, D.P., Peng, J. (2005-12-01). Loss of function of def selectively up-regulates Δ113p53 expression to arrest expansion growth of digestive organs in zebrafish. Genes and Development 19 (23) : 2900-2911. ScholarBank@NUS Repository.
Abstract: Transcription factor p53 forms a network with associated factors to regulate the cell cycle and apoptosis in response to environmental stresses. However, there is currently no direct genetic evidence to show if or how the p53 pathway functions during organogenesis. Here we present evidence to show that the zebrafish def (digestive-organ expansion factor) gene encodes a novel pan-endoderm-specific factor. A loss-of-function mutation in def confers hypoplastic digestive organs and selectively up-regulates the expression of Δ113p53, counterpart to a newly identified isoform of p53 produced by an alternative internal promoter in intron 4 of the p53 gene in human. The increased Δ113p53 expression is limited to within the mutant digestive organs, and this increase selectively induces the expression of p53-responsive genes to trigger the arrest of the cell cycle but not apoptosis, resulting in compromised organ growth in the mutant. Our data demonstrate that, while induction of expression of p53 and/or its isoforms is crucial to suppress abnormal cell growth, Δ113p53 is tightly regulated by an organ/tissue-specific factor Def, especially during organogenesis, to prevent adverse inhibition of organ/tissue growth. © 2005 by Cold Spring Harbor Laboratory Press.
Source Title: Genes and Development
ISSN: 08909369
DOI: 10.1101/gad.1366405
Appears in Collections:Staff Publications

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