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Title: Isolation and characterisation of a serum lectin from blue gourami, Trichogaster trichopterus(Pallus)
Authors: Fock, W.L.
Chen, C.L. 
Lam, T.J. 
Sin, Y.M. 
Keywords: Affinity chromatography
Blood cells
Blue gourami
Colony forming unit (cfu)
Fish immunity
Fish lectin
Protein purification
Trichogaster trichopterus (Pallus)
Issue Date: Aug-2000
Citation: Fock, W.L.,Chen, C.L.,Lam, T.J.,Sin, Y.M. (2000-08). Isolation and characterisation of a serum lectin from blue gourami, Trichogaster trichopterus(Pallus). Fish and Shellfish Immunology 10 (6) : 489-504. ScholarBank@NUS Repository.
Abstract: A novel lectin, designated BGL, has been purified from the serum of blue gourami, Trichogaster trichopterus, with the use of (NH4)2SO4fractionation, affinity chromatography and gel filtration chromatography. Electrophoretic analyses and mass spectrometric study of purified BGL showed that the lectin is composed of two isoforms with native molecular masses estimated to be 65 and 66kDa, and two subunits of 32 and 34kDa on SDS-PAGE under non-reducing conditions. Upon reduction with 20mm dithiothreitol (DTT), BGL showed two close bands of 27 and 29kDa. After isoelectric focusing, the lectin focused as close double bands at pH5·6. The N-termini of both isoforms share the same sequence (HGEENRXGPR) and show no significant homology with any known proteins. The BGL agglutinating activity is specifically inhibited by N-acetyl-D-galactosamine and N-acetyl-D-glucosamine, and to a lesser degree by D-(+)-mannose, but not by D-(+)-galactose, D-(+)-glucose, maltose or N-acetyl-D-mannosamine. Haemagglutination assay showed that BGL is more specific for rabbit than mouse, chicken, rat or guinea pig erythrocytes, and haemagglutination was Ca2+-dependent. In addition, BGL could agglutinate a range of micro-organisms and yeast cells, with the exception of some fish pathogens, such as Aeromonas hydrophila (strains: PPD 134/91 and PPD 11/90) and Vibrio harveyi (strain: W618). Localisation of BGL by fluorescein isothiocyanate (FITC)-labelled antibodies revealed that the lectin is associated with the cell surface of fish leukocytes. © 2000 Academic Press.
Source Title: Fish and Shellfish Immunology
ISSN: 10504648
Appears in Collections:Staff Publications

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