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Title: Irditoxin, a novel covalently linked heterodimeric three-finger toxin with high taxon-specific neurotoxicity
Authors: Pawlak, J.
Mackessy, S.P.
Sixberry, N.M.
Stura, E.A.
Le Du, M.H.
Méenez, R.
Foo, C.S.
Ménez, A.
Nirthanan, S.
Kini, R.M. 
Keywords: Acetylcholine receptor
Neuromuscular junction
Postsynaptic neurotoxin
Snake venom
Issue Date: Feb-2009
Citation: Pawlak, J., Mackessy, S.P., Sixberry, N.M., Stura, E.A., Le Du, M.H., Méenez, R., Foo, C.S., Ménez, A., Nirthanan, S., Kini, R.M. (2009-02). Irditoxin, a novel covalently linked heterodimeric three-finger toxin with high taxon-specific neurotoxicity. FASEB Journal 23 (2) : 534-545. ScholarBank@NUS Repository.
Abstract: A novel heterodimeric three-finger neurotoxin, irditoxin, was isolated from venom of the brown treesnake Boiga irregularis (Colubridae). Irditoxin subunit amino acid sequences were determined by Edman degradation and cDNA sequencing. The crystal structure revealed two subunits with a three-finger protein fold, typical for "nonconventional" toxins such as denmotoxin, bucandin, and candoxin. This is the first colubrid three- finger toxin dimer, covalently connected via an interchain disulfide bond. Irditoxin showed taxon-specific lethality toward birds and lizards and was nontoxic toward mice. It produced a potent neuromuscular blockade at the avian neuromuscular junction (IC 50=10 nM), comparable to α-bungarotoxin, but was three orders of magnitude less effective at the mammalian neuromuscular junction. Co- valently linked heterodimeric three-finger toxins found in colubrid venoms constitute a new class of venom pep- tides, which may be a useful source of new neurobiology probes and therapeutic leads. © FASEB.
Source Title: FASEB Journal
ISSN: 08926638
DOI: 10.1096/fj.08-113555
Appears in Collections:Staff Publications

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