Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0003-9861(02)00630-6
DC FieldValue
dc.titleInteraction of wheat high-mobility-group proteins with four-way-junction DNA and characterization of the structure and expression of HMGA gene
dc.contributor.authorZhang, W.
dc.contributor.authorWu, Q.
dc.contributor.authorPwee, K.-H.
dc.contributor.authorManjunatha Kini, R.
dc.date.accessioned2014-10-27T08:31:53Z
dc.date.available2014-10-27T08:31:53Z
dc.date.issued2003-01-15
dc.identifier.citationZhang, W., Wu, Q., Pwee, K.-H., Manjunatha Kini, R. (2003-01-15). Interaction of wheat high-mobility-group proteins with four-way-junction DNA and characterization of the structure and expression of HMGA gene. Archives of Biochemistry and Biophysics 409 (2) : 357-366. ScholarBank@NUS Repository. https://doi.org/10.1016/S0003-9861(02)00630-6
dc.identifier.issn00039861
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100950
dc.description.abstractPlant high-mobility-group (HMG) chromosomal proteins are the most abundant and ubiquitous nonhistone proteins found in the nuclei of higher eukaryotes. There are only two families of HMG proteins, namely, HMGA and HMGB in plants. The cDNA encoding wheat HMGa protein was isolated and characterized. Wheat HMGA cDNA encodes a protein of 189 amino acid residues. At its N terminus, there is a histone H1-like structure, which is a common feature of plant HMGA proteins, followed by four AT-hook motifs. Polymerase chain reaction results show that the gene contains a single intron of 134 bp. All four AT-hook motifs are encoded by the second exon. Northern blot results show that the expression of HMGA gene is much higher in organs undergoing active cell proliferation. Gel retardation analysis show that wheat HMGa, b, c and histone H1 bind to four-way-junction DNA with high binding affinity, but affinity is dramatically reduced with increasing Mg2+ and Na+ ion concentration. Competition binding studies show that proteins share overlapping binding sites on four-way-junction DNA. HMGd does not bind to four-way-junction DNA. © 2002 Elsevier Science (USA). All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0003-9861(02)00630-6
dc.sourceScopus
dc.subjectCell proliferation
dc.subjectFour-way-junction DNA
dc.subjectHMG proteins
dc.subjectProtein-DNA interaction
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0003-9861(02)00630-6
dc.description.sourcetitleArchives of Biochemistry and Biophysics
dc.description.volume409
dc.description.issue2
dc.description.page357-366
dc.description.codenABBIA
dc.identifier.isiut000180314100013
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

27
checked on May 18, 2022

WEB OF SCIENCETM
Citations

25
checked on May 18, 2022

Page view(s)

112
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.