Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.70.2.954-960.2004
Title: Insecticidal Bacillus thuringiensis Silences Erwinia carotovora Virulence by a New Form of Microbial Antagonism, Signal Interference
Authors: Dong, Y.-H.
Zhang, X.-F.
Xu, J.-L.
Zhang, L.-H. 
Issue Date: Feb-2004
Citation: Dong, Y.-H., Zhang, X.-F., Xu, J.-L., Zhang, L.-H. (2004-02). Insecticidal Bacillus thuringiensis Silences Erwinia carotovora Virulence by a New Form of Microbial Antagonism, Signal Interference. Applied and Environmental Microbiology 70 (2) : 954-960. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.70.2.954-960.2004
Abstract: It is commonly known that bacteria may produce antibiotics to interfere with the normal biological functions of their competitors in order to gain competitive advantages. Here we report that Bacillus thuringiensis suppressed the quorum-sensing-dependent virulence of plant pathogen Erwinia carotovora through a new form of microbial antagonism, signal interference. E. carotovora produces and responds to acyl-homoserine lactone (AHL) quorum-sensing signals to regulate antibiotic production and expression of virulence genes, whereas B. thuringiensis strains possess AHL-lactonase, which is a potent AHL-degrading enzyme. B. thuringiensis did not seem to interfere with the normal growth of E. carotovora; rather, it abolished the accumulation of AHL signal when they were cocultured. In planta, B. thuringiensis significantly decreased the incidence of E. carotovora infection and symptom development of potato soft rot caused by the pathogen. The biocontrol efficiency is correlated with the ability of bacterial strains to produce AHL-lactonase. While all the seven AHL-lactonase-producing B. thuringiensis strains provided significant protection against E. carotovora infection, Bacillus fusiformis and Escherichia coli strains that do not process AHL-degradation enzyme showed little effect in biocontrol. Mutation of aiiA, the gene encoding AHL-lactonase in B. thuringiensis, resulted in a substantial decrease in biocontrol efficacy. These results suggest that signal interference mechanisms existing in natural ecosystems could be explored as a new version of antagonism for prevention of bacterial infections.
Source Title: Applied and Environmental Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/100938
ISSN: 00992240
DOI: 10.1128/AEM.70.2.954-960.2004
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

146
checked on Aug 13, 2019

WEB OF SCIENCETM
Citations

132
checked on Aug 6, 2019

Page view(s)

46
checked on Aug 17, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.