Please use this identifier to cite or link to this item: https://doi.org/10.1002/ijc.1428
Title: Inhibition of angiopoietin-1 expression in tumor cells by an antisense RNA approach inhibited xenograft tumor growth in immunodeficient mice
Authors: Shim, W.S.N.
Teh, M.
Mack, P.O.P.
Ge, R. 
Keywords: Angiopoietin-1
Anti-angiogenesis
Antisense RNA
Cancer therapy
Tumor angiogenesis
Issue Date: 1-Oct-2001
Citation: Shim, W.S.N., Teh, M., Mack, P.O.P., Ge, R. (2001-10-01). Inhibition of angiopoietin-1 expression in tumor cells by an antisense RNA approach inhibited xenograft tumor growth in immunodeficient mice. International Journal of Cancer 94 (1) : 6-15. ScholarBank@NUS Repository. https://doi.org/10.1002/ijc.1428
Abstract: Angiopoietin-1 (Ang1) is an angiogenic growth factor that functions through activation of its endothelium-specific tyrosine kinase receptor Tie2; it mediates the interaction between endothelial and surrounding cells to promote the remodeling, maturation and stabilization of blood vessels. Although Ang1 is expressed constitutively in many adult tissues, its role in tumor growth and metastasis is not clear. Here we describe experiments in which Ang1 expression was inhibited in HeLa cells by an antisense RNA approach. The modified HeLa cells produced significantly less Ang1 protein both in cultured cells and in tumors formed when these cells were injected into immunodeficient mice. The Ang1 antisense tumors grew much more slowly, with significantly reduced tumor angiogenesis compared with control tumors. Furthermore, they also had substantially increased tumor cell apoptosis and decreased tumor necrosis. Our results indicate that the perturbation of Ang1 expression in tumors could be an effective method to control tumor growth by inhibiting tumor angiogenesis and that antisense RNA is an efficient way to inhibit Ang1 protein production in tumor cells. © 2001 Wiley-Liss, Inc.
Source Title: International Journal of Cancer
URI: http://scholarbank.nus.edu.sg/handle/10635/100929
ISSN: 00207136
DOI: 10.1002/ijc.1428
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