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|Title:||Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth||Authors:||Li, B.
Neurite outgrowth guidance
|Issue Date:||Aug-2005||Citation:||Li, B., Ma, Y., Wang, S., Moran, P.M. (2005-08). Influence of carboxyl group density on neuron cell attachment and differentiation behavior: Gradient-guided neurite outgrowth. Biomaterials 26 (24) : 4956-4963. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2005.01.018||Abstract:||A UV pre-irradiation step followed by a UV grafting step was used to graft poly(acrylic acid) (PAA) on polymeric substrates. These substrates were then used to investigate the influence of carboxyl groups (-COOH) on cell behavior. Both the attachment and differentiation behaviors of C17.2 cells showed a -COOH group density-dependent response. In order to achieve an even distribution of cells on a -COOH gradient surface for neuron differentiation studies, an Ar plasma post-treatment was applied to the PAA-grafted surfaces. It greatly improved the cell adhesion properties with little damage to -COOH groups. This allows uniform distributions of seeded cells even on substrates with -COOH gradients. A linear or stepped -COOH gradient was found to be capable of serving as a repelling cue to guide the outgrowth of neurites from C17.2 cells. Up to 3.7 times more cells developed neurites growing down the -COOH gradient than growing up it. © 2005 Elsevier Ltd. All rights reserved.||Source Title:||Biomaterials||URI:||http://scholarbank.nus.edu.sg/handle/10635/100926||ISSN:||01429612||DOI:||10.1016/j.biomaterials.2005.01.018|
|Appears in Collections:||Staff Publications|
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