Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100916
Title: Induced mutations in cassava using somatic embryos and the identification of mutant plants with altered starch yield and composition
Authors: Joseph, R.
Yeoh, H.-H. 
Loh, C.-S. 
Keywords: γ-Irradiation
Cassava
Manihot esculenta
Somatic embryos
Starch biosynthesis
Issue Date: Aug-2004
Citation: Joseph, R.,Yeoh, H.-H.,Loh, C.-S. (2004-08). Induced mutations in cassava using somatic embryos and the identification of mutant plants with altered starch yield and composition. Plant Cell Reports 23 (1-2) : 91-98. ScholarBank@NUS Repository.
Abstract: A cyclic somatic embryogenic system was used to induce mutations in cassava variety PRC 60a in vitro. Globular-stage somatic embryos were selected as suitable experimental materials, and 50 Gy of γ-rays was determined to be the optimal dose for inducing mutations. During subsequent field trials, more than 50% of the regenerated mutant lines varied morphologically from wild-type plants. Consequently, we used this approach to induce genetic variability for obtaining novel cassava cultivars. Among the different mutant lines obtained, lines S14 and S15 showed large morphological variations. In 10-month-old S14 and S15 mutant lines, storage root yield was reduced 17-fold and 60-fold, respectively, compared to wild-type plants, while the storage roots of S15 mutant plants also exhibited an almost 50% decrease in starch content and a significant reduction (30%) in amylose content. These two features were observed throughout the different developmental stages of the storage roots in S15 plants. © Springer-Verlag 2004.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/100916
ISSN: 07217714
Appears in Collections:Staff Publications

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