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Title: Immunostaining of germline stem cells and the niche in drosophila ovaries
Authors: Luo, L.
Chai, P.C.
Cai, Y. 
Keywords: Drosophila
Germline stem cells
Issue Date: 2013
Citation: Luo, L.,Chai, P.C.,Cai, Y. (2013). Immunostaining of germline stem cells and the niche in drosophila ovaries. Methods in Molecular Biology 1035 : 1-7. ScholarBank@NUS Repository.
Abstract: Stem cells have the ability to switch between proliferative (self-renewal) and differentiation modes. The Drosophila germarium is a well-established in vivo model for the study of communication between stem cells and their niche. One commonly used technique for such study is immunostaining that allows examination of protein localization at a fi xed time point. This chapter provides a detailed protocol for immunofl uorescence staining of Drosophila ovaries. This protocol has been optimized to enable explicit visualization of the niche structure, as well as to maximize the degree of multiplexing for protein labeling and detection. © Springer Science+Business Media, LLC 2013.
Source Title: Methods in Molecular Biology
ISBN: 9781627035071
ISSN: 10643745
DOI: 10.1007/978-1-62703-508-8-1
Appears in Collections:Staff Publications

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