Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aquaculture.2005.04.053
Title: Immune response of freshwater fish, guppy, Poicelia reticulata and gouramy, Trichogaster trichopterus to recombinant coat protein of Epinephelus tauvina nervous necrosis virus
Authors: Hegde, A.
Lam, T.J. 
Sin, Y.M.
Keywords: Gouramy
Guppy
Immune response
In vitro neutralization
Recombinant coat protein
Issue Date: 12-Sep-2005
Citation: Hegde, A., Lam, T.J., Sin, Y.M. (2005-09-12). Immune response of freshwater fish, guppy, Poicelia reticulata and gouramy, Trichogaster trichopterus to recombinant coat protein of Epinephelus tauvina nervous necrosis virus. Aquaculture 249 (1-4) : 77-84. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aquaculture.2005.04.053
Abstract: Immune response of guppy, Poicelia reticulata and gouramy, Trichogaster tricopterus towards recombinant coat protein of nervous necrosis virus was evaluated by immersion and injection immunization, respectively, and the response was compared with that against the formalin-inactivated Epinephelus tauvina nervous necrosis virus (ETNNV). In vitro neutralization of ETNNV and guppy nervous necrosis virus (GNNV) was performed to measure the neutralizing antibody response. In vitro neutralization using the whole-body extract of immersion immunized guppy and the antisera from injection immunized gouramy showed that the formalin-inactivated ETNNV, and its recombinant coat protein induce neutralizing antibody response in fish. In addition, the rabbit polyclonal antisera raised against the recombinant coat protein of ETNNV also neutralized the GNNV and ETNNV in vitro showing the importance of recombinant coat protein as a candidate vaccine against viral nervous necrosis and its suitability for immersion delivery. © 2005 Elsevier B.V. All rights reserved.
Source Title: Aquaculture
URI: http://scholarbank.nus.edu.sg/handle/10635/100886
ISSN: 00448486
DOI: 10.1016/j.aquaculture.2005.04.053
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