Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-59745-102-4-9
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dc.titleIdentification of plant virus IRES
dc.contributor.authorWong, S.-M.
dc.contributor.authorKoh, D.C.-Y.
dc.contributor.authorLiu, D.
dc.date.accessioned2014-10-27T08:31:02Z
dc.date.available2014-10-27T08:31:02Z
dc.date.issued2008
dc.identifier.citationWong, S.-M.,Koh, D.C.-Y.,Liu, D. (2008). Identification of plant virus IRES. Methods in Molecular Biology 451 : 125-133. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-59745-102-4-9" target="_blank">https://doi.org/10.1007/978-1-59745-102-4-9</a>
dc.identifier.isbn9781588298270
dc.identifier.issn10643745
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100874
dc.description.abstractAbstrat: Plant RNA viruses exploit nonorthodox strategies, such as the use of internal ribosomal entry sites (IRES), to express multiple genes from a single RNA species. IRES elements have been reported in tobacco etch virus (TEV), crucifer infecting tobamovirus (crTMV), hibiscus chlorotic ringspot virus (HCRSV), and many other animal and plant RNA viruses. In this chapter, the methodology used to identify and characterize a plant virus IRES element, including construction of a translation reporter vector for testing the IRES activity, testing the IRES activity in coupled in vitro transcription and translation systems and mammalian cells analysis of RNA stability, and sucrose gradient analysis and polysome profiling, is presented. © 2008 Humana Press, a part of Springer Science + Business Media, LLC.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-59745-102-4-9
dc.sourceScopus
dc.subjectGene expression
dc.subjectIRES
dc.subjectPlant virus
dc.subjectTranslation
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1007/978-1-59745-102-4-9
dc.description.sourcetitleMethods in Molecular Biology
dc.description.volume451
dc.description.page125-133
dc.identifier.isiutNOT_IN_WOS
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