Please use this identifier to cite or link to this item: https://doi.org/10.1021/pr1008916
Title: Identification of novel proteins from the venom of a cryptic snake drysdalia coronoides by a combined transcriptomics and proteom cs approach
Authors: Chatrath, S.T.
Chapeaurouge, A.
Lin, Q. 
Lim, T.K. 
Dunstan, N.
Mirtschin, P.
Kumar, P.P. 
Kini, R.M. 
Keywords: Phospholipases
Proteome
Tandem mass spectrometry
Three-finger toxins
Transcriptome
Issue Date: 4-Feb-2011
Citation: Chatrath, S.T., Chapeaurouge, A., Lin, Q., Lim, T.K., Dunstan, N., Mirtschin, P., Kumar, P.P., Kini, R.M. (2011-02-04). Identification of novel proteins from the venom of a cryptic snake drysdalia coronoides by a combined transcriptomics and proteom cs approach. Journal of Proteome Research 10 (2) : 739-750. ScholarBank@NUS Repository. https://doi.org/10.1021/pr1008916
Abstract: We have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the crude venom followed by HPLC separation of the resulting peptides and MALDI-TOF/TOF mass spectrometric analysis. Importantly, the tandem MS data of the tryptic peptides of the venom not only confirmed the predicted protein sequences deduced from the transcriptome, but also added to our knowledge about the venom composition through identification of two more toxin families. Using both the approaches, we were able to identify proteins belonging to eight different snake venom protein superfamilies, namely, three-finger toxins, serine protease inhibitors, cysteine rich secretory proteins, phospholipases A 2, venom nerve growth factors, snake venom metalloproteases, vespryns, and a new family phospholipase B. We also identified three novel proteins belonging to the three-finger toxin superfamily. © 2011 American Chemical Society.
Source Title: Journal of Proteome Research
URI: http://scholarbank.nus.edu.sg/handle/10635/100873
ISSN: 15353893
DOI: 10.1021/pr1008916
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