Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100872
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dc.titleIdentification of human, rat and mouse nocistatin in brain and human cerebrospinal fluid
dc.contributor.authorLee, T.-L.
dc.contributor.authorFung, F.M.Y.
dc.contributor.authorChen, F.-G.
dc.contributor.authorChou, N.
dc.contributor.authorOkuda-Ashitaka, E.
dc.contributor.authorIto, S.
dc.contributor.authorNishiuchi, Y.
dc.contributor.authorKimura, T.
dc.contributor.authorTachibana, S.
dc.date.accessioned2014-10-27T08:31:00Z
dc.date.available2014-10-27T08:31:00Z
dc.date.issued1999-05-14
dc.identifier.citationLee, T.-L.,Fung, F.M.Y.,Chen, F.-G.,Chou, N.,Okuda-Ashitaka, E.,Ito, S.,Nishiuchi, Y.,Kimura, T.,Tachibana, S. (1999-05-14). Identification of human, rat and mouse nocistatin in brain and human cerebrospinal fluid. NeuroReport 10 (7) : 1537-1541. ScholarBank@NUS Repository.
dc.identifier.issn09594965
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100872
dc.description.abstractNocistatin was recently isolated from bovine brain and shown to block hyperalgesia and allodynia induced by nociceptin and prostaglandin (PG) E2. The counterparts of human, rat and mouse are deduced from their precursor prepronociceptin to be 30, 35, and 41 residue peptide respectively. To identify these mature forms of nocistatin, three peptides were synthesized and a detection program for nocistatin was developed, using high pressure liquid chromatography (HPLC) along with specific radioimmunoassay (RIA). Nocistatin extracted from human, rat and mouse brain were subjected to HPLC and nocistatin-like immunoreactivity (NST-IR) was determined. All three species showed two NST-IR peaks, one of which coincided with that of the corresponding putative nocistatin. The same NST-IR was also detected in human cerebrospinal fluid (CSF).
dc.sourceScopus
dc.subjectCerebrospinal fluid
dc.subjectHuman brain
dc.subjectNociceptin
dc.subjectNocistatin
dc.subjectPain
dc.subjectRadioimmunoassay
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleNeuroReport
dc.description.volume10
dc.description.issue7
dc.description.page1537-1541
dc.description.codenNERPE
dc.identifier.isiutNOT_IN_WOS
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