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|Title:||Identification of differentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes||Authors:||Yin, Z.
Cyprinus carpio L.
Cytochrome C oxidase
|Issue Date:||1999||Citation:||Yin, Z., He, J.Y., Gong, Z., Lam, T.J., Sin, Y.M. (1999). Identification of differentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 124 (1) : 41-50. ScholarBank@NUS Repository. https://doi.org/10.1016/S0305-0491(99)00095-4||Abstract:||A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology search in Genbank (EMBL) revealed those differentially expressed cDNA clones encode for cytochrome c oxidase sub-unit II and III (COII and COIII), elongation factor-1β (EF-1β), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA). Copyright (C) 1999 Elsevier Science Inc.||Source Title:||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/100870||ISSN:||03050491||DOI:||10.1016/S0305-0491(99)00095-4|
|Appears in Collections:||Staff Publications|
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