Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0305-0491(99)00095-4
Title: Identification of differentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes
Authors: Yin, Z.
He, J.Y. 
Gong, Z. 
Lam, T.J. 
Sin, Y.M. 
Keywords: 16S rRNA
BH
Cyprinus carpio L.
Cytochrome C oxidase
Differential screening
EF-1β
HSC70
Mitogenesis
Issue Date: 1999
Citation: Yin, Z., He, J.Y., Gong, Z., Lam, T.J., Sin, Y.M. (1999). Identification of differentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 124 (1) : 41-50. ScholarBank@NUS Repository. https://doi.org/10.1016/S0305-0491(99)00095-4
Abstract: A cDNA library was constructed from the message RNA (mRNA) obtained from Con A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Differential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induced HK leucocytes of carp. The differential expression patterns of certain cDNA clones were confirmed by Southern-blot and Northern-blot analysis. Single-pass of the sequencing analysis and homology search in Genbank (EMBL) revealed those differentially expressed cDNA clones encode for cytochrome c oxidase sub-unit II and III (COII and COIII), elongation factor-1β (EF-1β), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA). Copyright (C) 1999 Elsevier Science Inc.
Source Title: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/100870
ISSN: 03050491
DOI: 10.1016/S0305-0491(99)00095-4
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