Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0048736
DC FieldValue
dc.titleIdentification of a Plant Viral RNA Genome in the Nucleus
dc.contributor.authorGao, R.
dc.contributor.authorLiu, P.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-10-27T08:30:57Z
dc.date.available2014-10-27T08:30:57Z
dc.date.issued2012-11-14
dc.identifier.citationGao, R., Liu, P., Wong, S.-M. (2012-11-14). Identification of a Plant Viral RNA Genome in the Nucleus. PLoS ONE 7 (11) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0048736
dc.identifier.issn19326203
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100868
dc.description.abstractViruses contain either DNA or RNA as genomes. DNA viruses replicate within nucleus, while most RNA viruses, especially (+)-sense single-stranded RNA, replicate and are present within cytoplasm. We proposed a new thought that is contrary to the common notion that (+)-sense single-stranded RNA viruses are present only in the cytoplasm. In this study, we question whether the genome of a plant RNA virus (non-retroviral) is present in the nucleus of infected cells? Hibiscus chlorotic ringspot virus (HCRSV) RNA was detected in the nucleus of infected cells, as shown by fluorescent in situ hybridization. Western blot using anti-histone 3 and anti-phosphoenolpyruvate carboxylase showed that nuclei were highly purified from mock and HCRSV-infected kenaf (Hibiscus cannabilis L.) leaves, respectively. The p23 and HCRSV coat protein (CP) coding regions were both amplified from total RNA extracted from isolated nuclei. Viral RNA in the nucleus may be used to generate viral microRNAs (vir-miRNAs), as five putative vir-miRNAs were predicted from HCRSV using the vir-miRNAs prediction database. The vir-miRNA (hcrsv-miR-H1-5p) was detected using TaqMan® stem-loop real-time PCR, and by northern blot using DIG-end labeled probe in HCRSV-infected kenaf leaves. Finally, a novel nuclear localization signal (NLS) was discovered in p23 of HCRSV. The NLS interacts with importin α and facilitates viral RNA genome to enter nucleus. We demonstrate the presence of a (+)-sense single-stranded viral RNA within nucleus. © 2012 Gao et al.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0048736
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pone.0048736
dc.description.sourcetitlePLoS ONE
dc.description.volume7
dc.description.issue11
dc.description.page-
dc.identifier.isiut000311151900037
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2012-identification_plant_viral_RNA_genome-pub.pdf710.8 kBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

21
checked on Nov 25, 2022

WEB OF SCIENCETM
Citations

19
checked on Nov 17, 2022

Page view(s)

397
checked on Nov 24, 2022

Download(s)

4
checked on Nov 24, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.