Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.M305322200
Title: Identification of a novel family of proteins in snake venoms. Purification and structural characterization of nawaprin from Naja nigricollis snake venom
Authors: Torres, A.M.
Wong, H.Y.
DESAI MALAN MANISH 
Moochhala, S.
Kuchel, P.W.
Kini, R.M. 
Issue Date: 10-Oct-2003
Citation: Torres, A.M., Wong, H.Y., DESAI MALAN MANISH, Moochhala, S., Kuchel, P.W., Kini, R.M. (2003-10-10). Identification of a novel family of proteins in snake venoms. Purification and structural characterization of nawaprin from Naja nigricollis snake venom. Journal of Biological Chemistry 278 (41) : 40097-40104. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M305322200
Abstract: The three-dimensional structure of nawaprin has been determined by nuclear magnetic resonance spectroscopy. This 51-amino acid residue peptide was isolated from the venom of the spitting cobra, Naja nigricollis, and is the first member of a new family of snake venom proteins referred to as waprins. Nawaprin is relatively flat and disc-like in shape, characterized by a spiral backbone configuration that forms outer and inner circular segments. The two circular segments are held together by four disulfide bonds, three of which are clustered at the base of the molecule. The inner segment contains a short antiparallel β-sheet, whereas the outer segment is devoid of secondary structures except for a small turn or 310 helix. The structure of nawaprin is very similar to elafin, a human leukocyte elastase-specific inhibitor. Although substantial parts of the nawaprin molecule are well defined, the tips of the outer and inner circular segments, which are hypothesized to be critical for binding interactions, are apparently disordered, similar to that found in elafin. The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/100866
ISSN: 00219258
DOI: 10.1074/jbc.M305322200
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

52
checked on Oct 6, 2022

WEB OF SCIENCETM
Citations

49
checked on Oct 6, 2022

Page view(s)

141
checked on Oct 6, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.