Please use this identifier to cite or link to this item: https://doi.org/10.1089/hum.2005.16.1404
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dc.titleHybrid cytomegalovirus enhancer-H1 promoter-based plasmid and baculovirus vectors mediate effective RNA interference
dc.contributor.authorOng, S.T.
dc.contributor.authorLi, F.
dc.contributor.authorDu, J.
dc.contributor.authorTan, Y.W.
dc.contributor.authorWang, S.
dc.date.accessioned2014-10-27T08:30:47Z
dc.date.available2014-10-27T08:30:47Z
dc.date.issued2005-12
dc.identifier.citationOng, S.T., Li, F., Du, J., Tan, Y.W., Wang, S. (2005-12). Hybrid cytomegalovirus enhancer-H1 promoter-based plasmid and baculovirus vectors mediate effective RNA interference. Human Gene Therapy 16 (12) : 1404-1412. ScholarBank@NUS Repository. https://doi.org/10.1089/hum.2005.16.1404
dc.identifier.issn10430342
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100852
dc.description.abstractPlasmid and viral vectors harboring an RNA polymerase (Pol) III promoter would be useful in achieving sustained cellular expression of short interfering RNA (siRNA) to inhibit disease-associated genes. Given that transcription machineries directed by certain Pol II and III promoters may use common factors, we investigated whether the enhancer of the Pol II cytomegalovirus (CMV) immediate-early promoter could improve the efficacy of RNA interference mediated by the Pol III H1 promoter. We constructed a hybrid promoter by appending the CMV enhancer 5′ to the H1 promoter. In the context of plasmid vectors, the hybrid promoter provided up to 50% greater inhibition of the expression of target genes than the unmodified H1 promoter and extended the silencing effect beyond that provided by the H1 promoter. Insect baculoviruses can infect a broad range of mammalian cell types. We constructed a baculovector expression cassette in which the synthesis of short hairpin RNA was under the control of the hybrid CMV enhancer-H1 promoter. This recombinant baculovirus vector was capable of suppressing expression of a target gene by 95% in cultured cells and by 82% in vivo in rat brain. These findings indicate that the hybrid CMV enhancer-H1 promoter can be used favorably for RNA interference. © Mary Ann Liebert, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/hum.2005.16.1404
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1089/hum.2005.16.1404
dc.description.sourcetitleHuman Gene Therapy
dc.description.volume16
dc.description.issue12
dc.description.page1404-1412
dc.description.codenHGTHE
dc.identifier.isiut000234253000005
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