Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100833
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dc.titleHigh-performance affinity capture-removal of bacterial pyrogen from solutions
dc.contributor.authorDing, J.L.
dc.contributor.authorZhu, Y.
dc.contributor.authorHo, B.
dc.date.accessioned2014-10-27T08:30:35Z
dc.date.available2014-10-27T08:30:35Z
dc.date.issued2001-08-15
dc.identifier.citationDing, J.L., Zhu, Y., Ho, B. (2001-08-15). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository.
dc.identifier.issn13872273
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100833
dc.description.abstractSynthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-4347(01)00227-4
dc.sourceScopus
dc.subjectAffinity adsorption
dc.subjectEndotoxins
dc.subjectLipopolysaccharides
dc.subjectPyrogen
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleJournal of Chromatography B: Biomedical Sciences and Applications
dc.description.volume759
dc.description.issue2
dc.description.page237-246
dc.description.codenJCBBE
dc.identifier.isiut000169915300005
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