Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100833
Title: High-performance affinity capture-removal of bacterial pyrogen from solutions
Authors: Ding, J.L. 
Zhu, Y. 
Ho, B.
Keywords: Affinity adsorption
Endotoxins
Lipopolysaccharides
Pyrogen
Issue Date: 15-Aug-2001
Citation: Ding, J.L., Zhu, Y., Ho, B. (2001-08-15). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository.
Abstract: Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.
Source Title: Journal of Chromatography B: Biomedical Sciences and Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/100833
ISSN: 13872273
Appears in Collections:Staff Publications

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