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|Title:||High-performance affinity capture-removal of bacterial pyrogen from solutions||Authors:||Ding, J.L.
|Issue Date:||15-Aug-2001||Citation:||Ding, J.L., Zhu, Y., Ho, B. (2001-08-15). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository.||Abstract:||Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.||Source Title:||Journal of Chromatography B: Biomedical Sciences and Applications||URI:||http://scholarbank.nus.edu.sg/handle/10635/100833||ISSN:||13872273|
|Appears in Collections:||Staff Publications|
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