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|Title:||High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro||Authors:||Cheng, P.-K.
|Issue Date:||2001||Citation:||Cheng, P.-K.,Lakshmanan, P.,Swarup, S. (2001). High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro. In Vitro Cellular and Developmental Biology - Plant 37 (5) : 592-598. ScholarBank@NUS Repository.||Abstract:||An in vitro method was developed for high-frequency shoot regeneration and continuous production of rapid-cycling B. oleracea in large numbers. The high regenerative capacity was tissue-dependent. Developmental polarity (apical and basal ends) of the explants appeared to play a regulatory role in shoot morphogenesis in this system. High-frequency shoot regeneration was obtained with N6-benzyladenine or thidiazuron-supplemented media. Delayed and reduced regenerative ability of cultures in air-tight vessels and the dramatic suppression of shoot regeneration in internodal explants by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid implicate a possible involvement of ethylene in shoot morphogenesis in this species. Rooting of regenerated shoots of B. oleracea occurred readily on α-naphthaleneacetic acid-supplemented media. Rooted plantlets were successfully established in soil and developed normal fertile flowers and viable seeds.||Source Title:||In Vitro Cellular and Developmental Biology - Plant||URI:||http://scholarbank.nus.edu.sg/handle/10635/100832||ISSN:||10545476|
|Appears in Collections:||Staff Publications|
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