Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100832
Title: High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro
Authors: Cheng, P.-K.
Lakshmanan, P. 
Swarup, S. 
Keywords: Ethylene
Morphogenesis
Plant development
Rapid-cycling brassica
Thidiazuron
Issue Date: 2001
Citation: Cheng, P.-K.,Lakshmanan, P.,Swarup, S. (2001). High-frequency direct shoot regeneration and continuous production of rapid-cycling Brassica oleracea in vitro. In Vitro Cellular and Developmental Biology - Plant 37 (5) : 592-598. ScholarBank@NUS Repository.
Abstract: An in vitro method was developed for high-frequency shoot regeneration and continuous production of rapid-cycling B. oleracea in large numbers. The high regenerative capacity was tissue-dependent. Developmental polarity (apical and basal ends) of the explants appeared to play a regulatory role in shoot morphogenesis in this system. High-frequency shoot regeneration was obtained with N6-benzyladenine or thidiazuron-supplemented media. Delayed and reduced regenerative ability of cultures in air-tight vessels and the dramatic suppression of shoot regeneration in internodal explants by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid implicate a possible involvement of ethylene in shoot morphogenesis in this species. Rooting of regenerated shoots of B. oleracea occurred readily on α-naphthaleneacetic acid-supplemented media. Rooted plantlets were successfully established in soil and developed normal fertile flowers and viable seeds.
Source Title: In Vitro Cellular and Developmental Biology - Plant
URI: http://scholarbank.nus.edu.sg/handle/10635/100832
ISSN: 10545476
Appears in Collections:Staff Publications

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