Please use this identifier to cite or link to this item: https://doi.org/10.1046/j.1365-313X.1997.11040703.x
Title: High mobility group proteins HMG-1 and MHG-I/Y bind to a positive regulatory region of the pea plastocyanin gene promoter
Authors: Webster, C.I.
Packman, L.C.
Pwee, K.-H. 
Gray, J.C.
Issue Date: Apr-1997
Citation: Webster, C.I., Packman, L.C., Pwee, K.-H., Gray, J.C. (1997-04). High mobility group proteins HMG-1 and MHG-I/Y bind to a positive regulatory region of the pea plastocyanin gene promoter. Plant Journal 11 (4) : 703-715. ScholarBank@NUS Repository. https://doi.org/10.1046/j.1365-313X.1997.11040703.x
Abstract: A 268 bp region (P268) of the pea plastocyanin gene promoter responsible for high-level expression has been shown to interact with the high mobility group proteins HMG-1 and HMG-I/Y isolated from pea shoot chromatin. cDNAs encoding an HMG-1 protein of 154 amino acid residues containing a single HMG-box and a C-terminal acidic tail and an HMG-I/Y-like protein of 197 amino acid residues containing four AT-hooks have been isolated and expressed in Escherichia coli to provide large amounts of full-length proteins. DNase I footprinting identified eight binding sites for HMG-I/Y and six binding sites for HMG-1 in P268. Inhibition of binding by the antibiotic distamycin, which binds in the minor groove of A/T-rich DNA, revealed that HMG-I/Y binding was 400-fold more sensitive than HMG-1 binding. Binding-site selection from a pool of random oligonucleotides indicated that HMG-I/Y binds to oligonucleotides containing stretches of five or more A/T bp and HMG-1 binds preferentially to oligonucleotides enriched in dinucleotides such as TpT and TpG.
Source Title: Plant Journal
URI: http://scholarbank.nus.edu.sg/handle/10635/100829
ISSN: 09607412
DOI: 10.1046/j.1365-313X.1997.11040703.x
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