Please use this identifier to cite or link to this item: https://doi.org/10.1002/dvdy.10051
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dc.titleGreen fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8
dc.contributor.authorGong, Z.
dc.contributor.authorJu, B.
dc.contributor.authorWang, X.
dc.contributor.authorHe, J.
dc.contributor.authorWan, H.
dc.contributor.authorSudha, P.M.
dc.contributor.authorYan, T.
dc.date.accessioned2014-10-27T08:29:44Z
dc.date.available2014-10-27T08:29:44Z
dc.date.issued2002
dc.identifier.citationGong, Z., Ju, B., Wang, X., He, J., Wan, H., Sudha, P.M., Yan, T. (2002). Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8. Developmental Dynamics 223 (2) : 204-215. ScholarBank@NUS Repository. https://doi.org/10.1002/dvdy.10051
dc.identifier.issn10588388
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100791
dc.description.abstractA zebrafish cDNA encoding a novel keratin protein was characterized and named keratin8, or krt8. krt8 expression was initiated at 4.5 hr postfertilization, immediately after the time of zygotic genome activation. The expression is limited to a single layer of envelope cells on the surface of embryos and, in later stages, it also appears in the innermost epithelial layer of the anterior- and posteriormost portions of the digestive tract. In adult, its expression was limited to the surface layer of stratified epithelial tissues, including skin epidermis and epithelia of mouth, pharynx, esophagus, and rectum but not in the gastral and intestinal epithelia. By using a 2.2-kb promoter from krt8, several stable green fluorescent protein (gfp) transgenic zebrafish lines were established. All of these transgenic lines displayed GFP expression in tissues mentioned above except for the rectum; therefore, the pattern of transgenic GFP expression is essentially identical to that of the endogenous krt8 mRNAs. krt8-GFP fusion protein was also expressed in zebrafish embryos under a ubiquitous promoter, and the fusion protein was capable of assembling into intermediate filaments only in the epithelia that normally expressed krt8 mRNAs, indicating the specificity of keratin assembly in vivo. © 2002 Wiley-Liss, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/dvdy.10051
dc.sourceScopus
dc.subjectCytokeratin
dc.subjectEsophagus
dc.subjectGFP-fusion protein
dc.subjectIntermediate filaments
dc.subjectIntestine
dc.subjectPharynx
dc.subjectRectum
dc.subjectSkin
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1002/dvdy.10051
dc.description.sourcetitleDevelopmental Dynamics
dc.description.volume223
dc.description.issue2
dc.description.page204-215
dc.description.codenDEDYE
dc.identifier.isiut000173635600004
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