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Title: Genotypic variability of de novo shoot morphogenesis of Brassica oleracea in vitro in response to ethylene inhibitors and putrescine
Authors: Pua, E.-C. 
Deng, X.
Koh, A.T.-C.
Keywords: Brassica oleracea
Shoot regeneration
Tissue culture
Issue Date: Oct-1999
Citation: Pua, E.-C.,Deng, X.,Koh, A.T.-C. (1999-10). Genotypic variability of de novo shoot morphogenesis of Brassica oleracea in vitro in response to ethylene inhibitors and putrescine. Journal of Plant Physiology 155 (4-5) : 598-605. ScholarBank@NUS Repository.
Abstract: Effects of ethylene inhibitors and exogenous putrescine on the shoot regeneration capacity of cultured hypocotyl explants from 18 cultivars of cabbage, cauliflower and broccoli were investigated. All cultivars were poorly regenerative on Murashige and Skoog's medium supplemented with 0.5-1 mg · L-1 α-naphthaleneacetic acid in combination with 1-4 mg · L-1 benzyladenine. Shoot regeneration of some cultivars was enhanced by addition of 10 μmol · L-1 AgNO3 to the medium. The presence of 10 μmol · L-1 aminoethoxyvinylglycine (AVG) or 10 mmol · L-1 exogenous putrescine was also beneficial to regeneration, whereas combining chemical treatment with vacuum infiltration had little effect. Explants of all three cabbage cultivars tested (, and ), when grown on AgNO3 medium, produced significantly more ethylene than control explants, but there was no difference in the content of free polyamines (putrescine, spermidine and spermine) in both tissues. On the contrary, explants grown in the presence of AVG emanated lower ethylene during the three-week culture period. Furthermore, these explants also showed a significantly higher free putrescine content up to 7-10 days of culture, although the difference in free spermidine and spermine between AVG-grown and control explants was negligible. The results support the hypothesis that ethylene produced by explants often inhibits shoot regeneration in vitro.
Source Title: Journal of Plant Physiology
ISSN: 01761617
Appears in Collections:Staff Publications

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