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Title: Gene transfer to dorsal root ganglia by intrathecal injection: Effects on regeneration of peripheral nerves
Authors: Wang, X.
Wang, C.
Zeng, J.
Xu, X.
Hwang, P.Y.K.
Yee, W.-C.
Ng, Y.-K.
Wang, S. 
Keywords: Dorsal root ganglion
Gene transfer
Nerve growth factor
Nerve regeneration
Issue Date: Aug-2005
Citation: Wang, X., Wang, C., Zeng, J., Xu, X., Hwang, P.Y.K., Yee, W.-C., Ng, Y.-K., Wang, S. (2005-08). Gene transfer to dorsal root ganglia by intrathecal injection: Effects on regeneration of peripheral nerves. Molecular Therapy 12 (2) : 314-320. ScholarBank@NUS Repository.
Abstract: Gene delivery to sensory neurons of the dorsal root ganglion (DRG) offers the prospect of developing new clinical interventions against peripheral nerve diseases and disorders. Here we show that genes can be transferred to rat DRG through lumbar intrathecal injection of delivery vectors into the cerebrospinal fluid. Genes could be transferred to DRG using polyethylenimine (PEI)/DNA complexes, Lipofectamine 2000/DNA complexes, adeno-associated virus vectors, or baculovirus vectors. We also show that nerve growth factor cDNA, delivered through lumbar intrathecal injection of PEI complexes, was able to improve regeneration of transected rat sciatic nerves. These data demonstrate the viability of using an intrathecal gene delivery approach for treating peripheral neuropathies. Copyright © The American Society of Gene Therapy.
Source Title: Molecular Therapy
ISSN: 15250016
DOI: 10.1016/j.ymthe.2005.03.032
Appears in Collections:Staff Publications

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