Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1021115816540
DC FieldValue
dc.titleFunctional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid
dc.contributor.authorYang, S.H.
dc.contributor.authorYu, H.
dc.contributor.authorGoh, C.J.
dc.date.accessioned2014-10-27T08:28:58Z
dc.date.available2014-10-27T08:28:58Z
dc.date.issued2003-01
dc.identifier.citationYang, S.H., Yu, H., Goh, C.J. (2003-01). Functional characterisation of a cytokinin oxidase gene DSCKX1 in Dendrobium orchid. Plant Molecular Biology 51 (2) : 237-248. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1021115816540
dc.identifier.issn01674412
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100720
dc.description.abstractCytokinin oxidase plays an important role in the cytokinin regulatory processes. We have cloned a novel putative cytokinin oxidase, DSCKX1 (Dendrobium Sonia cytokinin oxidase), by mRNA differential display from shoot apices of Dendrobium Sonia cultured in the presence of BA. The DSCKX1 gene appears to have three alternative splicing forms and its expression of DSCKX1 was induced in a tissue-specific manner by cytokinins. In transgenic orchid plants overexpressing DSCKX1, the elevated level of cytokinin oxidase activity was accompanied by a reduction of cytokinin content. These plants exhibited slow shoot growth with numerous and long roots in vitro. Their calli also showed decreased capability of shoot formation. Conversly, antisense transgenic plants showed rapid proliferation of shoots and inhibition of root growth combined with a higher endogenous cytokinin content than wild-type plants. Thus DSCKX1 appears to play an important role on cytokinin metabolism and the related developmental programmes in orchid.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1023/A:1021115816540
dc.sourceScopus
dc.subjectCytokinin
dc.subjectCytokinin oxidase
dc.subjectDendrobium orchid
dc.subjectDSCKX1
dc.subjectShoot/root development
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1023/A:1021115816540
dc.description.sourcetitlePlant Molecular Biology
dc.description.volume51
dc.description.issue2
dc.description.page237-248
dc.description.codenPMBID
dc.identifier.isiut000179324300009
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

51
checked on Oct 22, 2019

WEB OF SCIENCETM
Citations

42
checked on Oct 15, 2019

Page view(s)

40
checked on Oct 12, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.