Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0055474
DC FieldValue
dc.titleFluorescent Transgenic Zebrafish Tg(nkx2.2a:mEGFP) Provides a Highly Sensitive Monitoring Tool for Neurotoxins
dc.contributor.authorZhang, X.
dc.contributor.authorGong, Z.
dc.date.accessioned2014-10-27T08:28:46Z
dc.date.available2014-10-27T08:28:46Z
dc.date.issued2013-02-01
dc.identifier.citationZhang, X., Gong, Z. (2013-02-01). Fluorescent Transgenic Zebrafish Tg(nkx2.2a:mEGFP) Provides a Highly Sensitive Monitoring Tool for Neurotoxins. PLoS ONE 8 (2) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0055474
dc.identifier.issn19326203
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100701
dc.description.abstractPreviously a standard toxicological test termed as DarT (Danio rerio Teratogenic assay) using wild type zebrafish embryos has been established and it is widely applied in toxicological and chemical screenings. As an increasing number of fluorescent transgenic zebrafish lines with specific fluorescent protein expression specifically expressed in different organs and tissues, we envision that the fluorescent markers may provide more sensitive endpoints for monitoring chemical induced phenotypical changes. Here we employed Tg(nkx2.2a:mEGFP) transgenic zebrafish which have GFP expression in the central nervous system to investigate its potential for screening neurotoxic chemicals. Five potential neurotoxins (acetaminophen, atenolol, atrazine, ethanol and lindane) and one neuroprotectant (mefenamic acid) were tested. We found that the GFP-labeled ventral axons from trunk motoneurons, which were easily observed in live fry and measured for quantification, were a highly sensitive to all of the five neurotoxins and the length of axons was significantly reduced in fry which looked normal based on DarT endpoints at low concentrations of neurotoxins. Compared to the most sensitive endpoints of DarT, ventral axon marker could improve the detection limit of these neurotoxins by about 10 fold. In contrast, there was no improvement for detection of the mefenamic acid compared to all DarT endpoints. Thus, ventral axon lengths provide a convenient and measureable marker specifically for neurotoxins. Our study may open a new avenue to use other fluorescent transgenic zebrafish embryos/fry to develop sensitive and specific toxicological tests for different categories of chemicals. © 2013 Zhang, Gong.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1371/journal.pone.0055474
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1371/journal.pone.0055474
dc.description.sourcetitlePLoS ONE
dc.description.volume8
dc.description.issue2
dc.description.page-
dc.identifier.isiut000314597900039
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2013-fluorescent_transgenic_zebrafish_tg-pub.pdf1 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

28
checked on May 23, 2022

WEB OF SCIENCETM
Citations

25
checked on May 23, 2022

Page view(s)

346
checked on May 12, 2022

Download(s)

5
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.