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Title: Fish germ cells
Authors: Xu, H.Y. 
Li, M.Y. 
Gui, J.F.
Hong, Y.H. 
Keywords: Fish
Germ cell
Germ plasm
Reproductive technology
Issue Date: 2010
Citation: Xu, H.Y., Li, M.Y., Gui, J.F., Hong, Y.H. (2010). Fish germ cells. Science China Life Sciences 53 (4) : 435-446. ScholarBank@NUS Repository.
Abstract: Fish, like many other animals, have two major cell lineages, namely the germline and soma. The germ-soma separation is one of the earliest events of embryonic development. Germ cells can be specifically labeled and isolated for culture and transplantation, providing tools for reproduction of endangered species in close relatives, such as surrogate production of trout in salmon. Haploid cell cultures, such as medaka haploid embryonic stem cells have recently been obtained, which are capable of mimicking sperm to produce fertile offspring, upon nuclear being directly transferred into normal eggs. Such fish originated from a mosaic oocyte that had a haploid meiotic nucleus and a transplanted haploid mitotic cell culture nucleus. The first semi-cloned fish is Holly. Here we review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology. © Science China Press and Springer-Verlag Berlin Heidelberg 2010.
Source Title: Science China Life Sciences
ISSN: 16747305
DOI: 10.1007/s11427-010-0058-8
Appears in Collections:Staff Publications

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