Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.10800
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dc.titleExpression of Recombinant Vitellogenin in the Yeast Pichia pastoris
dc.contributor.authorDing, J.L.
dc.contributor.authorLim, E.H.
dc.contributor.authorLi, H.F.
dc.contributor.authorKumar, J.K.
dc.contributor.authorLee, S.L.
dc.contributor.authorLam, T.J.
dc.date.accessioned2014-10-27T08:28:08Z
dc.date.available2014-10-27T08:28:08Z
dc.date.issued2004-02-05
dc.identifier.citationDing, J.L., Lim, E.H., Li, H.F., Kumar, J.K., Lee, S.L., Lam, T.J. (2004-02-05). Expression of Recombinant Vitellogenin in the Yeast Pichia pastoris. Biotechnology and Bioengineering 85 (3) : 330-339. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.10800
dc.identifier.issn00063592
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100644
dc.description.abstractVitellogenin (Vtg) plays vital roles as precursor to the yolk proteins and as carrier for lipids, carbohydrates, phosphates, metal ions, vitamins, and hormones into the oocytes during the massive deposition of yolk nutrients for subsequent nourishment of the developing embryos. Reproductive success is highly sensitive to the nutritional quality of the broodstock diet, which greatly affects the egg and larval viability. We present a novel strategy for genetically engineering a Pichia pastoris yeast strain that constitutively produces recombinant Vtg (rVtg), for application as an enriched feed. The tilapia Oreochromis aureus Vtg (OaVtg) cDNA (5.3 kb) was cloned into a nonsecretory pGAPZA vector. Clones containing up to 31 copies of glyceraldehyde-3-phosphate dehydrogenase (GAP)-promoter-driven Vtg expression cassettes were isolated. These clones expressed a membrane-associated intracellular rVtg protein of 194 kDa, constituting up to 1.16% of total protein. To facilitate future purification of rVtg, we explored the possibility of secreting rVtg using the native Vtg secretion signal and the α-factor secretion signal of Saccharomyces cerevisiae. However, neither signal promoted the secretion of rVtg. The clones maximally expressed rVtg at 23°C, reaching a peak at 22 h in shake flasks and 16 h in a fermentor. The clones exhibited a significant increase in essential amino acids and long-chain polyunsaturated fatty acids, which are important for its application as a high-quality nutrient feed. © 2004 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/bit.10800
dc.sourceScopus
dc.subjectConstitutive expression
dc.subjectEnriched-nutrient feed
dc.subjectPichia pastoris
dc.subjectVitellogenin
dc.subjectYolk protein
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1002/bit.10800
dc.description.sourcetitleBiotechnology and Bioengineering
dc.description.volume85
dc.description.issue3
dc.description.page330-339
dc.description.codenBIBIA
dc.identifier.isiut000188706600012
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