Please use this identifier to cite or link to this item:
https://doi.org/10.1111/j.1095-8649.2008.01955.x
DC Field | Value | |
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dc.title | Enzymatic and mitochondrial responses to 5 months of aerial exposure in the slender lungfish Protopterus dolloi | |
dc.contributor.author | Staples, J.F. | |
dc.contributor.author | Kajimura, M. | |
dc.contributor.author | Wood, C.M. | |
dc.contributor.author | Patel, M. | |
dc.contributor.author | Ip, Y.K. | |
dc.contributor.author | McClelland, G.B. | |
dc.date.accessioned | 2014-10-27T08:27:32Z | |
dc.date.available | 2014-10-27T08:27:32Z | |
dc.date.issued | 2008-08 | |
dc.identifier.citation | Staples, J.F., Kajimura, M., Wood, C.M., Patel, M., Ip, Y.K., McClelland, G.B. (2008-08). Enzymatic and mitochondrial responses to 5 months of aerial exposure in the slender lungfish Protopterus dolloi. Journal of Fish Biology 73 (3) : 608-622. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1095-8649.2008.01955.x | |
dc.identifier.issn | 00221112 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/100591 | |
dc.description.abstract | Mitochondrial respiration and activities of key metabolic enzymes from liver and white skeletal muscle were compared between control aquatic slender lungfish Protopterus dolloi, and those exposed to air for 5 months. Activities of citrate synthase, glycogen phosphorylase, phosphofructokinase and pyruvate kinase in liver were not affected by air-exposure. In muscle, air-exposure reduced citrate synthase and pyruvate kinase activities (relative to tissue wet mass) by 63 and 50%, respectively. Liver carnitine palmitoyl transferase activity (relative to mitochondrial protein) decreased by half following air-exposure, but there was no change in muscle. In mitochondria isolated from muscle, state 3 and state 4 respiration were reduced by 74 and 89%, respectively following air-exposure, but liver mitochondria were not affected. In liver, air-exposure increased activities of ornithine-urea cycle enzymes including glutamine synthase, carbamoyl-phosphate synthase III and arginase, by 1.9- to 4.2-fold. Carbamoyl-phosphate synthase III activity could not be detected in muscle, indicating that urea is not synthesized in this tissue. These data suggest that skeletal muscle metabolism is downregulated in air-exposure, conserving energy and protein during a period when the animals cannot forage. In contrast, ATP production capacities in the liver are maintained, and this may permit expensive urea biosynthesis to continue during aerial exposure. © 2008 The Authors. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1095-8649.2008.01955.x | |
dc.source | Scopus | |
dc.subject | Aestivation | |
dc.subject | Dipnoi | |
dc.subject | Glycolysis | |
dc.subject | Ornithine-urea cycle | |
dc.subject | Oxidative phosphorylation | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1111/j.1095-8649.2008.01955.x | |
dc.description.sourcetitle | Journal of Fish Biology | |
dc.description.volume | 73 | |
dc.description.issue | 3 | |
dc.description.page | 608-622 | |
dc.description.coden | JFIBA | |
dc.identifier.isiut | 000258169800010 | |
Appears in Collections: | Staff Publications |
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